Existence of log-phase Escherichia coli persisters and lasting memory of a starvation pulse
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Existence of log-phase Escherichia coli persisters and lasting memory of a starvation pulse. / Svenningsen, Mikkel Skjoldan; Svenningsen, Sine Lo; Sørensen, Michael Askvad; Mitarai, Namiko.
I: Life Science Alliance, Bind 5, Nr. 2, e202101076, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Existence of log-phase Escherichia coli persisters and lasting memory of a starvation pulse
AU - Svenningsen, Mikkel Skjoldan
AU - Svenningsen, Sine Lo
AU - Sørensen, Michael Askvad
AU - Mitarai, Namiko
PY - 2022
Y1 - 2022
N2 - The vast majority of a bacterial population is killed when treated with a lethal concentration of antibiotics. The time scale of this killing is often comparable with the bacterial generation time before the addition of antibiotics. Yet, a small subpopulation typically survives for an extended period. However, the long-term killing dynamics of bacterial cells has not been fully quantified even in well-controlled laboratory conditions. We constructed a week-long killing assay and followed the survival fraction of Escherichia coli K12 exposed to a high concentration of ciprofloxacin. We found that long-term survivors were formed during exponential growth, with some cells surviving at least 7 d. The long-term dynamics contained at least three time scales, which greatly enhances predictions of the population survival time compared with the biphasic extrapolation from the short-term behavior. Furthermore, we observed a long memory effect of a brief starvation pulse, which was dependent on the (p)ppGpp synthase relA. Specifically, 1 h of carbon starvation before antibiotics exposure increased the surviving fraction by nearly 100-fold even after 4 d of ciprofloxacin treatment.
AB - The vast majority of a bacterial population is killed when treated with a lethal concentration of antibiotics. The time scale of this killing is often comparable with the bacterial generation time before the addition of antibiotics. Yet, a small subpopulation typically survives for an extended period. However, the long-term killing dynamics of bacterial cells has not been fully quantified even in well-controlled laboratory conditions. We constructed a week-long killing assay and followed the survival fraction of Escherichia coli K12 exposed to a high concentration of ciprofloxacin. We found that long-term survivors were formed during exponential growth, with some cells surviving at least 7 d. The long-term dynamics contained at least three time scales, which greatly enhances predictions of the population survival time compared with the biphasic extrapolation from the short-term behavior. Furthermore, we observed a long memory effect of a brief starvation pulse, which was dependent on the (p)ppGpp synthase relA. Specifically, 1 h of carbon starvation before antibiotics exposure increased the surviving fraction by nearly 100-fold even after 4 d of ciprofloxacin treatment.
KW - BACTERIAL PERSISTENCE
KW - TOLERANCE
KW - STRINGENT
KW - GROWTH
KW - SYSTEM
KW - GENE
KW - RNA
U2 - 10.26508/lsa.202101076
DO - 10.26508/lsa.202101076
M3 - Journal article
C2 - 34795016
VL - 5
JO - Life Science Alliance
JF - Life Science Alliance
SN - 2575-1077
IS - 2
M1 - e202101076
ER -
ID: 286842931