Host-specific microbial communities in three sympatric North Sea sponges
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Host-specific microbial communities in three sympatric North Sea sponges. / Naim, Mohd Azrul; Morillo, Jose A.; Sørensen, Søren Johannes; Abu Al-Soud, Waleed; Smidt, Hauke; Sipkema, Detmer.
I: F E M S Microbiology Ecology, Bind 90, Nr. 2, 2014, s. 390-403.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Host-specific microbial communities in three sympatric North Sea sponges
AU - Naim, Mohd Azrul
AU - Morillo, Jose A.
AU - Sørensen, Søren Johannes
AU - Abu Al-Soud, Waleed
AU - Smidt, Hauke
AU - Sipkema, Detmer
N1 - This article is protected by copyright. All rights reserved.
PY - 2014
Y1 - 2014
N2 - The establishment of next generation technology sequencing has deepened our knowledge of marine sponge-associated microbiota with the identification of at least 32 phyla of bacteria and archaea from a large number of sponge species. In this study we assessed the diversity of the microbial communities hosted by three sympatric sponges living in a semi-enclosed North-Sea environment using pyrosequencing of bacterial and archaeal 16S ribosomal RNA gene fragments. The three sponges harbour species-specific communities each dominated by a different class of Proteobacteria. An α-proteobacterial Rhodobacter-like phylotype was confirmed as the predominant symbiont of Halichondria panicea. The microbial communities of Haliclona xena and Haliclona oculata are described for the first time in this study and are dominated by Gammaproteobacteria and Betaproteobacteria, respectively. Several common phylotypes belonging to Chlamydiae, TM6, Actinobacteria and Betaproteobacteria were detected in all sponge samples. A number of phylotypes of the phylum Chlamydiae were present at an unprecedentedly high relative abundance of up to 14.4% ± 1.4% of the total reads, which suggests an important ecological role in North Sea sponges. These Chlamydiae-affiliated OTUs may represent novel lineages at least at the genus level as they are only 86-92% similar to known sequences. This article is protected by copyright. All rights reserved.
AB - The establishment of next generation technology sequencing has deepened our knowledge of marine sponge-associated microbiota with the identification of at least 32 phyla of bacteria and archaea from a large number of sponge species. In this study we assessed the diversity of the microbial communities hosted by three sympatric sponges living in a semi-enclosed North-Sea environment using pyrosequencing of bacterial and archaeal 16S ribosomal RNA gene fragments. The three sponges harbour species-specific communities each dominated by a different class of Proteobacteria. An α-proteobacterial Rhodobacter-like phylotype was confirmed as the predominant symbiont of Halichondria panicea. The microbial communities of Haliclona xena and Haliclona oculata are described for the first time in this study and are dominated by Gammaproteobacteria and Betaproteobacteria, respectively. Several common phylotypes belonging to Chlamydiae, TM6, Actinobacteria and Betaproteobacteria were detected in all sponge samples. A number of phylotypes of the phylum Chlamydiae were present at an unprecedentedly high relative abundance of up to 14.4% ± 1.4% of the total reads, which suggests an important ecological role in North Sea sponges. These Chlamydiae-affiliated OTUs may represent novel lineages at least at the genus level as they are only 86-92% similar to known sequences. This article is protected by copyright. All rights reserved.
U2 - 10.1111/1574-6941.12400
DO - 10.1111/1574-6941.12400
M3 - Journal article
C2 - 25088929
VL - 90
SP - 390
EP - 403
JO - F E M S Microbiology Ecology
JF - F E M S Microbiology Ecology
SN - 0168-6496
IS - 2
ER -
ID: 120837719