Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes
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Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes. / Sørensen, Iben Schildt; Dandanell, Gert.
I: FEMS Microbiology Letters, Bind 154, Nr. 2, 1997, s. 173-180.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes
AU - Sørensen, Iben Schildt
AU - Dandanell, Gert
PY - 1997
Y1 - 1997
N2 - From a genomic library of Sulfolobus solfataricus DSM1617 we have isolated and identified the purEK locus. Two open reading frames are identified as homologs of the purE and purK purine biosynthetic genes in Escherichia coli. The C-terminus of purE overlaps with the N-terminus of purK. When either of the genes is expressed from an E. coli promoter they can complement the corresponding purE and purK mutations in E. coli. PurE seems to be more closely related to eubacteria than to other archaea and to eukaryotes. Also the purK gene, which has not yet been found in other archaea, is more closely related to eubacteria than to eukaryotes.
AB - From a genomic library of Sulfolobus solfataricus DSM1617 we have isolated and identified the purEK locus. Two open reading frames are identified as homologs of the purE and purK purine biosynthetic genes in Escherichia coli. The C-terminus of purE overlaps with the N-terminus of purK. When either of the genes is expressed from an E. coli promoter they can complement the corresponding purE and purK mutations in E. coli. PurE seems to be more closely related to eubacteria than to other archaea and to eukaryotes. Also the purK gene, which has not yet been found in other archaea, is more closely related to eubacteria than to eukaryotes.
M3 - Journal article
VL - 154
SP - 173
EP - 180
JO - F E M S Microbiology Letters
JF - F E M S Microbiology Letters
SN - 0378-1097
IS - 2
ER -
ID: 226053