Selecting Schizosaccharomyces pombe diploids
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an h− strain with an h90 mat2-Pi-102 strain can be used to prevent sporulation. When ade6-M210/ade6-M216 mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.
Originalsprog | Engelsk |
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Tidsskrift | Cold Spring Harbor Protocols |
Vol/bind | 2017 |
Udgave nummer | 7 |
Sider (fra-til) | 537-540 |
Antal sider | 4 |
ISSN | 1940-3402 |
DOI | |
Status | Udgivet - jul. 2017 |
ID: 181456120