TY - JOUR

T1 - Transcriptional profiling of extracellular amino acid sensing in Saccharomyces cerevisiae and the role of Stp 1 p and Stp2p

AU - Eckert-Boulet, Nadine

AU - Nielsen, Peter Stein

AU - Friis, Carsten

AU - Moreira dos Santos, Margarida

AU - Nielsen, Jens

AU - Kielland-Brandt, Morten C.

AU - Regenberg, Birgitte

PY - 2004/6/1

Y1 - 2004/6/1

N2 - S. cerevisiae responds to the presence of amino acids in the environment through the membrane-bound complex SPS, by altering transcription of several genes. Global transcription analysis shows that 46 genes are induced by L-citrulline. Under the given conditions there appears to be only one pathway for induction with L-citrulline, and this pathway is completely dependent on the SPS component, Ssy1p, and either of the transcription factors, Stp1p and Stp2p. Besides the effects on amino acid permease genes, an ssy1 and an stp1 stp2 mutant exhibit a number of other transcriptional phenotypes, such as increased expression of genes subject to nitrogen catabolite repression and genes involved in stress response. A group of genes involved in the upper part of the glycolysis, including those encoding hexose transporters Hxt4p, Hxt5p, Hxt6p, Hxt7p, hexokinase Hxk1p, glyceraldehyde 3-phosphate dehydrogenase Tdh1p and glucokinase (Glk1p), shows increased transcription levels in either or both of the mutants. Also, most of the structural genes involved in trehalose and glycogen synthesis and a few genes in the glyoxylate cycle and the pentose phosphate pathway are derepressed in the ssy1 and stp1 stp2 strains.

AB - S. cerevisiae responds to the presence of amino acids in the environment through the membrane-bound complex SPS, by altering transcription of several genes. Global transcription analysis shows that 46 genes are induced by L-citrulline. Under the given conditions there appears to be only one pathway for induction with L-citrulline, and this pathway is completely dependent on the SPS component, Ssy1p, and either of the transcription factors, Stp1p and Stp2p. Besides the effects on amino acid permease genes, an ssy1 and an stp1 stp2 mutant exhibit a number of other transcriptional phenotypes, such as increased expression of genes subject to nitrogen catabolite repression and genes involved in stress response. A group of genes involved in the upper part of the glycolysis, including those encoding hexose transporters Hxt4p, Hxt5p, Hxt6p, Hxt7p, hexokinase Hxk1p, glyceraldehyde 3-phosphate dehydrogenase Tdh1p and glucokinase (Glk1p), shows increased transcription levels in either or both of the mutants. Also, most of the structural genes involved in trehalose and glycogen synthesis and a few genes in the glyoxylate cycle and the pentose phosphate pathway are derepressed in the ssy1 and stp1 stp2 strains.

KW - Amino acid sensing

KW - NCR

KW - SSY1

KW - STP1

KW - STP2

KW - Transcription analysis

UR - http://www.scopus.com/inward/record.url?scp=3042725387&partnerID=8YFLogxK

U2 - 10.1002/yea.1120

DO - 10.1002/yea.1120

M3 - Journal article

C2 - 15197729

AN - SCOPUS:3042725387

VL - 21

SP - 635

EP - 648

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 8

ER -