A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2

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A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. / Garland, William; Comet, Itys; Wu, Mengjun; Radzisheuskaya, Aliaksandra; Rib, Leonor; Vitting-Seerup, Kristoffer; Lloret-Llinares, Marta; Sandelin, Albin; Helin, Kristian; Jensen, Torben Heick.

I: Cell Reports, Bind 29, Nr. 7, 2019, s. 1800-1811.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Garland, W, Comet, I, Wu, M, Radzisheuskaya, A, Rib, L, Vitting-Seerup, K, Lloret-Llinares, M, Sandelin, A, Helin, K & Jensen, TH 2019, 'A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2', Cell Reports, bind 29, nr. 7, s. 1800-1811. https://doi.org/10.1016/j.celrep.2019.10.011

APA

Garland, W., Comet, I., Wu, M., Radzisheuskaya, A., Rib, L., Vitting-Seerup, K., Lloret-Llinares, M., Sandelin, A., Helin, K., & Jensen, T. H. (2019). A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. Cell Reports, 29(7), 1800-1811. https://doi.org/10.1016/j.celrep.2019.10.011

Vancouver

Garland W, Comet I, Wu M, Radzisheuskaya A, Rib L, Vitting-Seerup K o.a. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. Cell Reports. 2019;29(7):1800-1811. https://doi.org/10.1016/j.celrep.2019.10.011

Author

Garland, William ; Comet, Itys ; Wu, Mengjun ; Radzisheuskaya, Aliaksandra ; Rib, Leonor ; Vitting-Seerup, Kristoffer ; Lloret-Llinares, Marta ; Sandelin, Albin ; Helin, Kristian ; Jensen, Torben Heick. / A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. I: Cell Reports. 2019 ; Bind 29, Nr. 7. s. 1800-1811.

Bibtex

@article{a44fc9a5a4ac4559b6253609697256f1,
title = "A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2",
abstract = "Pluripotent embryonic stem cells (ESCs) constitute an essential cellular niche sustained by epigenomic and transcriptional regulation. Any role of post-transcriptional processes remains less explored. Here, we identify a link between nuclear RNA levels, regulated by the poly(A) RNA exosome targeting (PAXT) connection, and transcriptional control by the polycomb repressive complex 2 (PRC2). Knockout of the PAXT component ZFC3H1 impairs mouse ESC differentiation. In addition to the upregulation of bona fide PAXT substrates, Zfc3h1-/- cells abnormally express developmental genes usually repressed by PRC2. Such de-repression is paralleled by decreased PRC2 binding to chromatin and low PRC2-directed H3K27 methylation. PRC2 complex stability is compromised in Zfc3h1-/- cells with elevated levels of unspecific RNA bound to PRC2 components. We propose that excess RNA hampers PRC2 function through its sequestration from DNA. Our results highlight the importance of balancing nuclear RNA levels and demonstrate the capacity of bulk RNA to regulate chromatin-associated proteins.",
author = "William Garland and Itys Comet and Mengjun Wu and Aliaksandra Radzisheuskaya and Leonor Rib and Kristoffer Vitting-Seerup and Marta Lloret-Llinares and Albin Sandelin and Kristian Helin and Jensen, {Torben Heick}",
note = "Copyright {\textcopyright} 2019 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2019",
doi = "10.1016/j.celrep.2019.10.011",
language = "English",
volume = "29",
pages = "1800--1811",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "7",

}

RIS

TY - JOUR

T1 - A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2

AU - Garland, William

AU - Comet, Itys

AU - Wu, Mengjun

AU - Radzisheuskaya, Aliaksandra

AU - Rib, Leonor

AU - Vitting-Seerup, Kristoffer

AU - Lloret-Llinares, Marta

AU - Sandelin, Albin

AU - Helin, Kristian

AU - Jensen, Torben Heick

N1 - Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2019

Y1 - 2019

N2 - Pluripotent embryonic stem cells (ESCs) constitute an essential cellular niche sustained by epigenomic and transcriptional regulation. Any role of post-transcriptional processes remains less explored. Here, we identify a link between nuclear RNA levels, regulated by the poly(A) RNA exosome targeting (PAXT) connection, and transcriptional control by the polycomb repressive complex 2 (PRC2). Knockout of the PAXT component ZFC3H1 impairs mouse ESC differentiation. In addition to the upregulation of bona fide PAXT substrates, Zfc3h1-/- cells abnormally express developmental genes usually repressed by PRC2. Such de-repression is paralleled by decreased PRC2 binding to chromatin and low PRC2-directed H3K27 methylation. PRC2 complex stability is compromised in Zfc3h1-/- cells with elevated levels of unspecific RNA bound to PRC2 components. We propose that excess RNA hampers PRC2 function through its sequestration from DNA. Our results highlight the importance of balancing nuclear RNA levels and demonstrate the capacity of bulk RNA to regulate chromatin-associated proteins.

AB - Pluripotent embryonic stem cells (ESCs) constitute an essential cellular niche sustained by epigenomic and transcriptional regulation. Any role of post-transcriptional processes remains less explored. Here, we identify a link between nuclear RNA levels, regulated by the poly(A) RNA exosome targeting (PAXT) connection, and transcriptional control by the polycomb repressive complex 2 (PRC2). Knockout of the PAXT component ZFC3H1 impairs mouse ESC differentiation. In addition to the upregulation of bona fide PAXT substrates, Zfc3h1-/- cells abnormally express developmental genes usually repressed by PRC2. Such de-repression is paralleled by decreased PRC2 binding to chromatin and low PRC2-directed H3K27 methylation. PRC2 complex stability is compromised in Zfc3h1-/- cells with elevated levels of unspecific RNA bound to PRC2 components. We propose that excess RNA hampers PRC2 function through its sequestration from DNA. Our results highlight the importance of balancing nuclear RNA levels and demonstrate the capacity of bulk RNA to regulate chromatin-associated proteins.

U2 - 10.1016/j.celrep.2019.10.011

DO - 10.1016/j.celrep.2019.10.011

M3 - Journal article

C2 - 31722198

VL - 29

SP - 1800

EP - 1811

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 7

ER -

ID: 230434493