A genome and single-nucleus cerebral cortex transcriptome atlas of the short-finned pilot whale Globicephala macrorhynchus

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  • Hui Kang
  • Liu, Qun
  • Inge Seim
  • Wenwei Zhang
  • Hanbo Li
  • Haiyu Gao
  • Wenzhi Lin
  • Mingli Lin
  • Peijun Zhang
  • Yaolei Zhang
  • Haoyang Gao
  • Yang Wang
  • Qin, Yating
  • Mingming Liu
  • Lijun Dong
  • Zixin Yang
  • Yingying Zhang
  • Lei Han
  • Guangyi Fan
  • Songhai Li
Cetaceans (dolphins, whales, and porpoises) have large and anatomically sophisticated brains. To expand our understanding of the cellular makeup of cetacean brains and the similarities and divergence between the brains of cetaceans and terrestrial mammals, we report a short-finned pilot whale (Globicephala macrorhynchus) single-nucleus transcriptome atlas. To achieve this goal, we assembled a chromosome-scale reference genome spanning 2.25 Gb on 22 chromosomes and profiled the gene expression of five major anatomical cortical regions of the short-finned pilot whale by single-nucleus RNA-sequencing (snRNA-seq). We identified six major cell lineages in the cerebral cortex (excitatory neurons, inhibitory neurons, oligodendrocytes, oligodendrocyte precursor cells, astrocytes, and endothelial cells), eight molecularly distinct subclusters of excitatory neurons, and four subclusters of inhibitory neurons. Finally, a comparison of snRNA-seq data from the short-finned pilot whale, human, and rhesus macaque revealed a broadly conserved cellular makeup of brain cell types. Our study provides genomic resources and molecular insights into cetacean brain evolution.
TidsskriftMolecular Ecology Resources
Udgave nummer5
Antal sider16
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
We are very grateful to Luka Culibrk and Dr Steven Jones from Canada's Michael Smith Genome Sciences Centre for providing the long‐finned pilot whale assembly, and all colleagues and students for their assistance during sampling. We thank Fujian Tan for providing technical assistance and useful discussions. This study was financially supported by Hainan Province Science and Technology Special Fund (no. ZDKJ2019011 to S.L.), the National Natural Science Foundation of China (nos. 42225604 and 41422604 to S.L.), the “One Belt and One Road” Science and Technology Cooperation Special Programme of the International Partnership Programme of the Chinese Academy of Sciences (no. 183446KYSB20200016 to S.L.), the Key Deployment Project of Centre for Ocean Mega‐Science of the Chinese Academy of Sciences (no. COMS2020Q15 to S.L.), the Specially‐Appointed Professor Programme of Jiangsu Province (to I.S.), the Jiangsu Foreign Expert Bureau (to I.S.), and the Jiangsu Provincial Department of Technology (grant JSSCTD202142, to I.S.).

Publisher Copyright:
© 2023 John Wiley & Sons Ltd.

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