An integrated linkage map reveals candidate genes underlying adaptive variation in Chinook salmon (Oncorhynchus tshawytscha)
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An integrated linkage map reveals candidate genes underlying adaptive variation in Chinook salmon (Oncorhynchus tshawytscha). / Mckinney, G. J.; Seeb, L. W.; Larson, W. A.; Gomez-Uchida, D.; Limborg, Morten Tønsberg; Brieuc, M. S. O.; Everett, M. V.; Naish, K. A.; Waples, R. K.; Seeb, J. E.
I: Molecular Ecology Resources, Bind 16, Nr. 3, 2016, s. 769-783.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - An integrated linkage map reveals candidate genes underlying adaptive variation in Chinook salmon (Oncorhynchus tshawytscha)
AU - Mckinney, G. J.
AU - Seeb, L. W.
AU - Larson, W. A.
AU - Gomez-Uchida, D.
AU - Limborg, Morten Tønsberg
AU - Brieuc, M. S. O.
AU - Everett, M. V.
AU - Naish, K. A.
AU - Waples, R. K.
AU - Seeb, J. E.
PY - 2016
Y1 - 2016
N2 - Salmonids are an important cultural and ecological resource exhibiting near worldwide distribution between their native and introduced range. Previous research has generated linkage maps and genomic resources for several species as well as genome assemblies for two species. We first leveraged improvements in mapping and genotyping methods to create a dense linkage map for Chinook salmon Oncorhynchus tshawytscha by assembling family data from different sources. We successfully mapped 14 620 SNP loci including 2336 paralogs in subtelomeric regions. This improved map was then used as a foundation to integrate genomic resources for gene annotation and population genomic analyses. We anchored a total of 286 scaffolds from the Atlantic salmon genome to the linkage map to provide a framework for the placement 11 728 Chinook salmon ESTs. Previously identified thermotolerance QTL were found to colocalize with several candidate genes including HSP70, a gene known to be involved in thermal response, as well as its inhibitor. Multiple regions of the genome with elevated divergence between populations were also identified, and annotation of ESTs in these regions identified candidate genes for fitness related traits such as stress response, growth and behaviour. Collectively, these results demonstrate the utility of combining genomic resources with linkage maps to enhance evolutionary inferences.
AB - Salmonids are an important cultural and ecological resource exhibiting near worldwide distribution between their native and introduced range. Previous research has generated linkage maps and genomic resources for several species as well as genome assemblies for two species. We first leveraged improvements in mapping and genotyping methods to create a dense linkage map for Chinook salmon Oncorhynchus tshawytscha by assembling family data from different sources. We successfully mapped 14 620 SNP loci including 2336 paralogs in subtelomeric regions. This improved map was then used as a foundation to integrate genomic resources for gene annotation and population genomic analyses. We anchored a total of 286 scaffolds from the Atlantic salmon genome to the linkage map to provide a framework for the placement 11 728 Chinook salmon ESTs. Previously identified thermotolerance QTL were found to colocalize with several candidate genes including HSP70, a gene known to be involved in thermal response, as well as its inhibitor. Multiple regions of the genome with elevated divergence between populations were also identified, and annotation of ESTs in these regions identified candidate genes for fitness related traits such as stress response, growth and behaviour. Collectively, these results demonstrate the utility of combining genomic resources with linkage maps to enhance evolutionary inferences.
KW - Adaptation
KW - Duplicated genome
KW - Linkage mapping
KW - Population differentiation
KW - RAD sequence
U2 - 10.1111/1755-0998.12479
DO - 10.1111/1755-0998.12479
M3 - Journal article
C2 - 26490135
AN - SCOPUS:84962886293
VL - 16
SP - 769
EP - 783
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 3
ER -
ID: 160799285