Analysis of primary cilia in directional cell migration in fibroblasts
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Analysis of primary cilia in directional cell migration in fibroblasts. / Christensen, Søren Tvorup; Veland, Iben; Schwab, Albrecht; Cammer, Michael; Satir, Peter.
I: Methods in Enzymology, Bind 525, 2013, s. 45-58.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Analysis of primary cilia in directional cell migration in fibroblasts
AU - Christensen, Søren Tvorup
AU - Veland, Iben
AU - Schwab, Albrecht
AU - Cammer, Michael
AU - Satir, Peter
N1 - Chapter Three in Cilia, Part B
PY - 2013
Y1 - 2013
N2 - Early studies of migrating fibroblasts showed that primary cilia orient in front of the nucleus and point toward the leading edge. Recent work has shown that primary cilia coordinate a series of signaling pathways critical to fibroblast cell migration during development and in wound healing. In particular, platelet-derived growth factor receptor alpha (PDGFRα) is compartmentalized to the primary cilium to activate signaling pathways that regulate reorganization of the cytoskeleton required for lamellipodium formation and directional migration in the presence of a specific ligand gradient. We summarize selected methods in analyzing ciliary function in directional cell migration, including immunofluorescence microscopy, scratch assay, and chemotaxis assay by micropipette addition of PDGFRα ligands to cultures of fibroblasts. These methods should be useful not only in studying cell migration but also more generally in delineating response pathways in cells with primary cilia.
AB - Early studies of migrating fibroblasts showed that primary cilia orient in front of the nucleus and point toward the leading edge. Recent work has shown that primary cilia coordinate a series of signaling pathways critical to fibroblast cell migration during development and in wound healing. In particular, platelet-derived growth factor receptor alpha (PDGFRα) is compartmentalized to the primary cilium to activate signaling pathways that regulate reorganization of the cytoskeleton required for lamellipodium formation and directional migration in the presence of a specific ligand gradient. We summarize selected methods in analyzing ciliary function in directional cell migration, including immunofluorescence microscopy, scratch assay, and chemotaxis assay by micropipette addition of PDGFRα ligands to cultures of fibroblasts. These methods should be useful not only in studying cell migration but also more generally in delineating response pathways in cells with primary cilia.
U2 - 10.1016/B978-0-12-397944-5.00003-1
DO - 10.1016/B978-0-12-397944-5.00003-1
M3 - Journal article
C2 - 23522464
VL - 525
SP - 45
EP - 58
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
ER -
ID: 47925268