Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes

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Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes. / Valen, Eivind; Preker, Pascal; Andersen, Peter Refsing; Zhao, Xiaobei; Chen, Yun; Ender, Christine; Dueck, Anne; Meister, Gunter; Sandelin, Albin Gustav; Jensen, Torben Heick.

I: Nature Structural and Molecular Biology, Bind 18, Nr. 9, 2011, s. 1075–1082.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Valen, E, Preker, P, Andersen, PR, Zhao, X, Chen, Y, Ender, C, Dueck, A, Meister, G, Sandelin, AG & Jensen, TH 2011, 'Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes', Nature Structural and Molecular Biology, bind 18, nr. 9, s. 1075–1082. https://doi.org/10.1038/nsmb.2091

APA

Valen, E., Preker, P., Andersen, P. R., Zhao, X., Chen, Y., Ender, C., Dueck, A., Meister, G., Sandelin, A. G., & Jensen, T. H. (2011). Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes. Nature Structural and Molecular Biology, 18(9), 1075–1082. https://doi.org/10.1038/nsmb.2091

Vancouver

Valen E, Preker P, Andersen PR, Zhao X, Chen Y, Ender C o.a. Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes. Nature Structural and Molecular Biology. 2011;18(9):1075–1082. https://doi.org/10.1038/nsmb.2091

Author

Valen, Eivind ; Preker, Pascal ; Andersen, Peter Refsing ; Zhao, Xiaobei ; Chen, Yun ; Ender, Christine ; Dueck, Anne ; Meister, Gunter ; Sandelin, Albin Gustav ; Jensen, Torben Heick. / Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes. I: Nature Structural and Molecular Biology. 2011 ; Bind 18, Nr. 9. s. 1075–1082.

Bibtex

@article{3f7920d104d540e98b196372452bd6a5,
title = "Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes",
abstract = "Efforts to catalog eukaryotic transcripts have uncovered many small RNAs (sRNAs) derived from gene termini and splice sites. Their biogenesis pathways are largely unknown, but a mechanism based on backtracking of RNA polymerase II (RNAPII) has been suggested. By sequencing transcripts 12-100 nucleotides in length from cells depleted of major RNA degradation enzymes and RNAs associated with Argonaute (AGO1/2) effector proteins, we provide mechanistic models for sRNA production. We suggest that neither splice site-associated (SSa) nor transcription start site-associated (TSSa) RNAs arise from RNAPII backtracking. Instead, SSa RNAs are largely degradation products of splicing intermediates, whereas TSSa RNAs probably derive from nascent RNAs protected by stalled RNAPII against nucleolysis. We also reveal new AGO1/2-associated RNAs derived from 3' ends of introns and from mRNA 3' UTRs that appear to draw from noncanonical microRNA biogenesis pathways.",
author = "Eivind Valen and Pascal Preker and Andersen, {Peter Refsing} and Xiaobei Zhao and Yun Chen and Christine Ender and Anne Dueck and Gunter Meister and Sandelin, {Albin Gustav} and Jensen, {Torben Heick}",
year = "2011",
doi = "10.1038/nsmb.2091",
language = "English",
volume = "18",
pages = "1075–1082",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "nature publishing group",
number = "9",

}

RIS

TY - JOUR

T1 - Biogenic mechanisms and utilization of small RNAs derived from human protein-coding genes

AU - Valen, Eivind

AU - Preker, Pascal

AU - Andersen, Peter Refsing

AU - Zhao, Xiaobei

AU - Chen, Yun

AU - Ender, Christine

AU - Dueck, Anne

AU - Meister, Gunter

AU - Sandelin, Albin Gustav

AU - Jensen, Torben Heick

PY - 2011

Y1 - 2011

N2 - Efforts to catalog eukaryotic transcripts have uncovered many small RNAs (sRNAs) derived from gene termini and splice sites. Their biogenesis pathways are largely unknown, but a mechanism based on backtracking of RNA polymerase II (RNAPII) has been suggested. By sequencing transcripts 12-100 nucleotides in length from cells depleted of major RNA degradation enzymes and RNAs associated with Argonaute (AGO1/2) effector proteins, we provide mechanistic models for sRNA production. We suggest that neither splice site-associated (SSa) nor transcription start site-associated (TSSa) RNAs arise from RNAPII backtracking. Instead, SSa RNAs are largely degradation products of splicing intermediates, whereas TSSa RNAs probably derive from nascent RNAs protected by stalled RNAPII against nucleolysis. We also reveal new AGO1/2-associated RNAs derived from 3' ends of introns and from mRNA 3' UTRs that appear to draw from noncanonical microRNA biogenesis pathways.

AB - Efforts to catalog eukaryotic transcripts have uncovered many small RNAs (sRNAs) derived from gene termini and splice sites. Their biogenesis pathways are largely unknown, but a mechanism based on backtracking of RNA polymerase II (RNAPII) has been suggested. By sequencing transcripts 12-100 nucleotides in length from cells depleted of major RNA degradation enzymes and RNAs associated with Argonaute (AGO1/2) effector proteins, we provide mechanistic models for sRNA production. We suggest that neither splice site-associated (SSa) nor transcription start site-associated (TSSa) RNAs arise from RNAPII backtracking. Instead, SSa RNAs are largely degradation products of splicing intermediates, whereas TSSa RNAs probably derive from nascent RNAs protected by stalled RNAPII against nucleolysis. We also reveal new AGO1/2-associated RNAs derived from 3' ends of introns and from mRNA 3' UTRs that appear to draw from noncanonical microRNA biogenesis pathways.

U2 - 10.1038/nsmb.2091

DO - 10.1038/nsmb.2091

M3 - Journal article

C2 - 21822281

VL - 18

SP - 1075

EP - 1082

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 9

ER -

ID: 33877164