Break-induced ATR and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast

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  • Jennifer Moss
  • Helen Tinline-Purvis
  • Carol A Walker
  • Lisa K Folkes
  • Michael R Stratford
  • Jacqueline Hayles
  • Kwang-Lae Hoe
  • Dong-Uk Kim
  • Han-Oh Park
  • Stephen E Kearsey
  • Oliver Fleck
  • Holmberg, Christian
  • Nielsen, Olaf
  • Timothy C Humphrey
Nucleotide synthesis is a universal response to DNA damage, but how this response facilitates DNA repair and cell survival is unclear. Here we establish a role for DNA damage-induced nucleotide synthesis in homologous recombination (HR) repair in fission yeast. Using a genetic screen, we found the Ddb1-Cul4(Cdt)² ubiquitin ligase complex and ribonucleotide reductase (RNR) to be required for HR repair of a DNA double-strand break (DSB). The Ddb1-Cul4(Cdt)² ubiquitin ligase complex is required for degradation of Spd1, an inhibitor of RNR in fission yeast. Accordingly, deleting spd1(+) suppressed the DNA damage sensitivity and the reduced HR efficiency associated with loss of ddb1(+) or cdt2(+). Furthermore, we demonstrate a role for nucleotide synthesis in postsynaptic gap filling of resected ssDNA ends during HR repair. Finally, we define a role for Rad3 (ATR) in nucleotide synthesis and HR through increasing Cdt2 nuclear levels in response to DNA damage. Our findings support a model in which break-induced Rad3 and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent Spd1 degradation and RNR activation promotes postsynaptic ssDNA gap filling during HR repair.
OriginalsprogEngelsk
TidsskriftGenes & Development
Vol/bind24
Udgave nummer23
Sider (fra-til)2705-16
Antal sider12
ISSN0890-9369
DOI
StatusUdgivet - 1 dec. 2010

ID: 33576476