Chitin and stress induced protein kinase activation
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.
Originalsprog | Engelsk |
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Titel | Plant pattern recognition receptors : methods and protocols |
Redaktører | Libo Shan, Ping He |
Antal sider | 10 |
Forlag | Springer |
Publikationsdato | 2017 |
Sider | 185-194 |
Kapitel | 15 |
ISBN (Trykt) | 978-1-4939-6858-9 |
ISBN (Elektronisk) | 978-1-4939-6859-6 |
DOI | |
Status | Udgivet - 2017 |
Navn | Methods in Molecular Biology |
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Vol/bind | 1578 |
ISSN | 1064-3745 |
ID: 181454851