Comparative genomics of Stutzerimonas balearica (Pseudomonas balearica): diversity, habitats, and biodegradation of aromatic compounds

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  • Francisco Salvà-Serra
  • Danilo Pérez-Pantoja
  • Raúl A. Donoso
  • Daniel Jaén-Luchoro
  • Fernandez Juarez, Victor
  • Hedvig Engström-Jakobsson
  • Edward R.B. Moore
  • Jorge Lalucat
  • Antoni Bennasar-Figueras
Stutzerimonas balearica (Pseudomonas balearica) has been found principally in oil-polluted environments. The capability of S. balearica to thrive from the degradation of pollutant compounds makes it a species of interest for potential bioremediation applications. However, little has been reported about the diversity of S. balearica. In this study, genome sequences of S. balearica strains from different origins were analyzed, revealing that it is a diverse species with an open pan-genome that will continue revealing new genes and functionalities as the genomes of more strains are sequenced. The nucleotide signatures and intra- and inter-species variation of the 16S rRNA genes of S. balearica were reevaluated. A strategy of screening 16S rRNA gene sequences in public databases enabled the detection of 158 additional strains, of which only 23% were described as S. balearica. The species was detected from a wide range of environments, although mostly from aquatic and polluted environments, predominantly related to petroleum oil. Genomic and phenotypic analyses confirmed that S. balearica possesses varied inherent capabilities for aromatic compounds degradation. This study increases the knowledge of the biology and diversity of S. balearica and will serve as a basis for future work with the species.
OriginalsprogEngelsk
Artikelnummer1159176
TidsskriftFrontiers in Microbiology
Vol/bind14
Antal sider19
ISSN1664-302X
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This work was supported by the Spanish MINECO through projects CGL2009-12180 and Consolider CSD2009-00006, as well as funds for competitive research groups from the Government of the Balearic Islands (the last two funds with FEDER co-funding). The DNA sequencing and analytical work was funded, in part, by the CCUG Project: Genomics and Proteomics Research on Bacterial Diversity. The CCUG is supported by the Department of Clinical Microbiology, Sahlgrenska University Hospital and the Sahlgrenska Academy of the University of Gothenburg, Sweden. DP-P and RD acknowledge the support of the FONDECYT 1201741, FONDECYT 11220354, and ANID-PIA/BASAL FB0002 grants of the Chilean government, and the LE19-05 project supported by the Fund of Scientific and Technological Equipment, year 2019, Universidad Tecnológica Metropolitana.

Funding Information:
The authors acknowledge the staff at the Culture Collection University of Gothenburg (CCUG) for maintaining and providing the strains. Max Häggblom and Norberto Palleroni (in memoriam), at the Department of Biochemistry and Microbiology (Rutgers University, NJ, United States), are acknowledged for providing strain st101 (= CCUG 66667). Shokouh Ghafari, at the Infectious Diseases Research Center (Birjand University of Medical Sciences, Birjand, Iran), is acknowledged for providing strain Z8 (= CCUG 70972). Anders Malmborg and the staff at the Substrate Department of the Department of Clinical Microbiology, Sahlgrenska University Hospital, are acknowledged for media preparation. The computations were partially performed on resources provided by the Swedish National Infrastructure for Computing (SNIC) through the Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX) under project SNIC 2019/8-176.

Funding Information:
The authors acknowledge the staff at the Culture Collection University of Gothenburg (CCUG) for maintaining and providing the strains. Max Häggblom and Norberto Palleroni (in memoriam), at the Department of Biochemistry and Microbiology (Rutgers University, NJ, United States), are acknowledged for providing strain st101 (= CCUG 66667). Shokouh Ghafari, at the Infectious Diseases Research Center (Birjand University of Medical Sciences, Birjand, Iran), is acknowledged for providing strain Z8 (= CCUG 70972). Anders Malmborg and the staff at the Substrate Department of the Department of Clinical Microbiology, Sahlgrenska University Hospital, are acknowledged for media preparation. The computations were partially performed on resources provided by the Swedish National Infrastructure for Computing (SNIC) through the Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX) under project SNIC 2019/8-176.

Publisher Copyright:
Copyright © 2023 Salvà-Serra, Pérez-Pantoja, Donoso, Jaén-Luchoro, Fernández-Juárez, Engström-Jakobsson, Moore, Lalucat and Bennasar-Figueras.

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