Compatibility of X-ray computed tomography with plant gene expression, rhizosphere bacterial communities and enzyme activities

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Compatibility of X-ray computed tomography with plant gene expression, rhizosphere bacterial communities and enzyme activities. / Ganther, Minh; Yim, Bunlong; Ibrahim, Zeeshan; Bienert, Manuela Desiree; Lippold, Eva; Maccario, Lorrie; Sørensen, Søren Johannes; Bienert, Gerd Patrick; Vetterlein, Doris; Heintz-Buschart, Anna; Blagodatskaya, Evgenia; Smalla, Kornelia; Tarkka, Mika T.

I: Journal of Experimental Botany, Bind 71, Nr. 18, 2020, s. 5603-5614.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ganther, M, Yim, B, Ibrahim, Z, Bienert, MD, Lippold, E, Maccario, L, Sørensen, SJ, Bienert, GP, Vetterlein, D, Heintz-Buschart, A, Blagodatskaya, E, Smalla, K & Tarkka, MT 2020, 'Compatibility of X-ray computed tomography with plant gene expression, rhizosphere bacterial communities and enzyme activities', Journal of Experimental Botany, bind 71, nr. 18, s. 5603-5614. https://doi.org/10.1093/jxb/eraa262

APA

Ganther, M., Yim, B., Ibrahim, Z., Bienert, M. D., Lippold, E., Maccario, L., Sørensen, S. J., Bienert, G. P., Vetterlein, D., Heintz-Buschart, A., Blagodatskaya, E., Smalla, K., & Tarkka, M. T. (2020). Compatibility of X-ray computed tomography with plant gene expression, rhizosphere bacterial communities and enzyme activities. Journal of Experimental Botany, 71(18), 5603-5614. https://doi.org/10.1093/jxb/eraa262

Vancouver

Ganther M, Yim B, Ibrahim Z, Bienert MD, Lippold E, Maccario L o.a. Compatibility of X-ray computed tomography with plant gene expression, rhizosphere bacterial communities and enzyme activities. Journal of Experimental Botany. 2020;71(18):5603-5614. https://doi.org/10.1093/jxb/eraa262

Author

Ganther, Minh ; Yim, Bunlong ; Ibrahim, Zeeshan ; Bienert, Manuela Desiree ; Lippold, Eva ; Maccario, Lorrie ; Sørensen, Søren Johannes ; Bienert, Gerd Patrick ; Vetterlein, Doris ; Heintz-Buschart, Anna ; Blagodatskaya, Evgenia ; Smalla, Kornelia ; Tarkka, Mika T. / Compatibility of X-ray computed tomography with plant gene expression, rhizosphere bacterial communities and enzyme activities. I: Journal of Experimental Botany. 2020 ; Bind 71, Nr. 18. s. 5603-5614.

Bibtex

@article{f650cff95bf0486eadc34afe1f322185,
title = "Compatibility of X-ray computed tomography with plant gene expression, rhizosphere bacterial communities and enzyme activities",
abstract = "Non-invasive X-ray computed tomography (XRCT) is increasingly used in rhizosphere research to visualize development of soil-root interfaces in situ. However, exposing living systems to X-rays can potentially impact their processes and metabolites. In order to evaluate these effects, we assessed the responses of rhizosphere processes 1 and 24 h after a low X-ray exposure (0.81 Gy). Changes in root gene expression patterns occurred 1 h after exposure with down-regulation of cell wall-, lipid metabolism-, and cell stress-related genes, but no differences remained after 24 h. At either time point, XRCT did not affect either root antioxidative enzyme activities or the composition of the rhizosphere bacterial microbiome and microbial growth parameters. The potential activities of leucine aminopeptidase and phosphomonoesterase were lower at 1 h, but did not differ from the control 24 h after exposure. A time delay of 24 h after a low X-ray exposure (0.81 Gy) was sufficient to reverse any effects on the observed rhizosphere systems. Our data suggest that before implementing novel experimental designs involving XRCT, a study on its impact on the investigated processes should be conducted.",
keywords = "Bacterial community composition, Extracellular soil enzyme, Maize, Plant elemental composition, Rhizosphere, Root gene expression, X-ray computed tomography",
author = "Minh Ganther and Bunlong Yim and Zeeshan Ibrahim and Bienert, {Manuela Desiree} and Eva Lippold and Lorrie Maccario and S{\o}rensen, {S{\o}ren Johannes} and Bienert, {Gerd Patrick} and Doris Vetterlein and Anna Heintz-Buschart and Evgenia Blagodatskaya and Kornelia Smalla and Tarkka, {Mika T.}",
year = "2020",
doi = "10.1093/jxb/eraa262",
language = "English",
volume = "71",
pages = "5603--5614",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "18",

}

RIS

TY - JOUR

T1 - Compatibility of X-ray computed tomography with plant gene expression, rhizosphere bacterial communities and enzyme activities

AU - Ganther, Minh

AU - Yim, Bunlong

AU - Ibrahim, Zeeshan

AU - Bienert, Manuela Desiree

AU - Lippold, Eva

AU - Maccario, Lorrie

AU - Sørensen, Søren Johannes

AU - Bienert, Gerd Patrick

AU - Vetterlein, Doris

AU - Heintz-Buschart, Anna

AU - Blagodatskaya, Evgenia

AU - Smalla, Kornelia

AU - Tarkka, Mika T.

PY - 2020

Y1 - 2020

N2 - Non-invasive X-ray computed tomography (XRCT) is increasingly used in rhizosphere research to visualize development of soil-root interfaces in situ. However, exposing living systems to X-rays can potentially impact their processes and metabolites. In order to evaluate these effects, we assessed the responses of rhizosphere processes 1 and 24 h after a low X-ray exposure (0.81 Gy). Changes in root gene expression patterns occurred 1 h after exposure with down-regulation of cell wall-, lipid metabolism-, and cell stress-related genes, but no differences remained after 24 h. At either time point, XRCT did not affect either root antioxidative enzyme activities or the composition of the rhizosphere bacterial microbiome and microbial growth parameters. The potential activities of leucine aminopeptidase and phosphomonoesterase were lower at 1 h, but did not differ from the control 24 h after exposure. A time delay of 24 h after a low X-ray exposure (0.81 Gy) was sufficient to reverse any effects on the observed rhizosphere systems. Our data suggest that before implementing novel experimental designs involving XRCT, a study on its impact on the investigated processes should be conducted.

AB - Non-invasive X-ray computed tomography (XRCT) is increasingly used in rhizosphere research to visualize development of soil-root interfaces in situ. However, exposing living systems to X-rays can potentially impact their processes and metabolites. In order to evaluate these effects, we assessed the responses of rhizosphere processes 1 and 24 h after a low X-ray exposure (0.81 Gy). Changes in root gene expression patterns occurred 1 h after exposure with down-regulation of cell wall-, lipid metabolism-, and cell stress-related genes, but no differences remained after 24 h. At either time point, XRCT did not affect either root antioxidative enzyme activities or the composition of the rhizosphere bacterial microbiome and microbial growth parameters. The potential activities of leucine aminopeptidase and phosphomonoesterase were lower at 1 h, but did not differ from the control 24 h after exposure. A time delay of 24 h after a low X-ray exposure (0.81 Gy) was sufficient to reverse any effects on the observed rhizosphere systems. Our data suggest that before implementing novel experimental designs involving XRCT, a study on its impact on the investigated processes should be conducted.

KW - Bacterial community composition

KW - Extracellular soil enzyme

KW - Maize

KW - Plant elemental composition

KW - Rhizosphere

KW - Root gene expression

KW - X-ray computed tomography

U2 - 10.1093/jxb/eraa262

DO - 10.1093/jxb/eraa262

M3 - Journal article

C2 - 32463450

AN - SCOPUS:85089139979

VL - 71

SP - 5603

EP - 5614

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 18

ER -

ID: 258332014