Comprehensive profiling of the fission yeast transcription start site activity during stress and media response
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Comprehensive profiling of the fission yeast transcription start site activity during stress and media response. / Thodberg, Malte; Thieffry, Axel; Bornholdt, Jette; Boyd, Mette; Holmberg, Christian; Azad, Ajuna; Workman, Christopher T; Chen, Yun; Ekwall, Karl; Nielsen, Olaf; Sandelin, Albin.
I: Nucleic Acids Research, Bind 47, Nr. 4, 28.02.2019, s. 1671-1691.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Comprehensive profiling of the fission yeast transcription start site activity during stress and media response
AU - Thodberg, Malte
AU - Thieffry, Axel
AU - Bornholdt, Jette
AU - Boyd, Mette
AU - Holmberg, Christian
AU - Azad, Ajuna
AU - Workman, Christopher T
AU - Chen, Yun
AU - Ekwall, Karl
AU - Nielsen, Olaf
AU - Sandelin, Albin
PY - 2019/2/28
Y1 - 2019/2/28
N2 - Fission yeast, Schizosaccharomyces pombe, is an attractive model organism for transcriptional and chromatin biology research. Such research is contingent on accurate annotation of transcription start sites (TSSs). However, comprehensive genome-wide maps of TSSs and their usage across commonly applied laboratory conditions and treatments for S. pombe are lacking. To this end, we profiled TSS activity genome-wide in S. pombe cultures exposed to heat shock, nitrogen starvation, hydrogen peroxide and two commonly applied media, YES and EMM2, using Cap Analysis of Gene Expression (CAGE). CAGE-based annotation of TSSs is substantially more accurate than existing PomBase annotation; on average, CAGE TSSs fall 50-75 bp downstream of PomBase TSSs and co-localize with nucleosome boundaries. In contrast to higher eukaryotes, dispersed TSS distributions are not common in S. pombe. Our data recapitulate known S. pombe stress expression response patterns and identify stress- and media-responsive alternative TSSs. Notably, alteration of growth medium induces changes of similar magnitude as some stressors. We show a link between nucleosome occupancy and genetic variation, and that the proximal promoter region is genetically diverse between S. pombe strains. Our detailed TSS map constitutes a central resource for S. pombe gene regulation research.
AB - Fission yeast, Schizosaccharomyces pombe, is an attractive model organism for transcriptional and chromatin biology research. Such research is contingent on accurate annotation of transcription start sites (TSSs). However, comprehensive genome-wide maps of TSSs and their usage across commonly applied laboratory conditions and treatments for S. pombe are lacking. To this end, we profiled TSS activity genome-wide in S. pombe cultures exposed to heat shock, nitrogen starvation, hydrogen peroxide and two commonly applied media, YES and EMM2, using Cap Analysis of Gene Expression (CAGE). CAGE-based annotation of TSSs is substantially more accurate than existing PomBase annotation; on average, CAGE TSSs fall 50-75 bp downstream of PomBase TSSs and co-localize with nucleosome boundaries. In contrast to higher eukaryotes, dispersed TSS distributions are not common in S. pombe. Our data recapitulate known S. pombe stress expression response patterns and identify stress- and media-responsive alternative TSSs. Notably, alteration of growth medium induces changes of similar magnitude as some stressors. We show a link between nucleosome occupancy and genetic variation, and that the proximal promoter region is genetically diverse between S. pombe strains. Our detailed TSS map constitutes a central resource for S. pombe gene regulation research.
U2 - 10.1093/nar/gky1227
DO - 10.1093/nar/gky1227
M3 - Journal article
C2 - 30566651
VL - 47
SP - 1671
EP - 1691
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 4
ER -
ID: 213858755