Detecting host responses to microbial stimulation using primary epithelial organoids

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Detecting host responses to microbial stimulation using primary epithelial organoids. / Bornholdt, Jette; Müller, Christina V.; Nielsen, Maria Juul; Strickertsson, Jesper; Rago, Daria; Chen, Yun; Maciag, Grzegorz; Skov, Jonathan; Wellejus, Anja; Schweiger, Pawel J.; Hansen, Stine L.; Broholm, Christa; Gögenur, Ismail; Maimets, Martti; Sloth, Stine; Hendel, Jakob; Baker, Adam; Sandelin, Albin; Jensen, Kim B.

I: Gut Microbes, Bind 15, Nr. 2, 2281012, 2023.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Bornholdt, J, Müller, CV, Nielsen, MJ, Strickertsson, J, Rago, D, Chen, Y, Maciag, G, Skov, J, Wellejus, A, Schweiger, PJ, Hansen, SL, Broholm, C, Gögenur, I, Maimets, M, Sloth, S, Hendel, J, Baker, A, Sandelin, A & Jensen, KB 2023, 'Detecting host responses to microbial stimulation using primary epithelial organoids', Gut Microbes, bind 15, nr. 2, 2281012. https://doi.org/10.1080/19490976.2023.2281012

APA

Bornholdt, J., Müller, C. V., Nielsen, M. J., Strickertsson, J., Rago, D., Chen, Y., Maciag, G., Skov, J., Wellejus, A., Schweiger, P. J., Hansen, S. L., Broholm, C., Gögenur, I., Maimets, M., Sloth, S., Hendel, J., Baker, A., Sandelin, A., & Jensen, K. B. (2023). Detecting host responses to microbial stimulation using primary epithelial organoids. Gut Microbes, 15(2), [2281012]. https://doi.org/10.1080/19490976.2023.2281012

Vancouver

Bornholdt J, Müller CV, Nielsen MJ, Strickertsson J, Rago D, Chen Y o.a. Detecting host responses to microbial stimulation using primary epithelial organoids. Gut Microbes. 2023;15(2). 2281012. https://doi.org/10.1080/19490976.2023.2281012

Author

Bornholdt, Jette ; Müller, Christina V. ; Nielsen, Maria Juul ; Strickertsson, Jesper ; Rago, Daria ; Chen, Yun ; Maciag, Grzegorz ; Skov, Jonathan ; Wellejus, Anja ; Schweiger, Pawel J. ; Hansen, Stine L. ; Broholm, Christa ; Gögenur, Ismail ; Maimets, Martti ; Sloth, Stine ; Hendel, Jakob ; Baker, Adam ; Sandelin, Albin ; Jensen, Kim B. / Detecting host responses to microbial stimulation using primary epithelial organoids. I: Gut Microbes. 2023 ; Bind 15, Nr. 2.

Bibtex

@article{5766bdf7398143b7a35df2fcd9343fee,

title = "Detecting host responses to microbial stimulation using primary epithelial organoids",

abstract = "The intestinal epithelium is constantly exposed to microbes residing in the lumen. Traditionally, the response to microbial interactions has been studied in cell lines derived from cancerous tissues, e.g. Caco-2. It is, however, unclear how the responses in these cancer cell lines reflect the responses of a normal epithelium and whether there might be microbial strain-specific effects. To address these questions, we derived organoids from the small intestine from a cohort of healthy individuals. Culturing intestinal epithelium on a flat laminin matrix induced their differentiation, facilitating analysis of microbial responses via the apical membrane normally exposed to the luminal content. Here, it was evident that the healthy epithelium across multiple individuals (n = 9) demonstrates robust acute both common and strain-specific responses to a range of probiotic bacterial strains (BB-12Ⓡ, LGGⓇ, DSM33361, and Bif195). Importantly, parallel experiments using the Caco-2 cell line provide no acute response. Collectively, we demonstrate that primary epithelial cells maintained as organoids represent a valuable resource for assessing interactions between the epithelium and luminal microbes across individuals, and that these models are likely to contribute to a better understanding of host microbe interactions.",

keywords = "bacterial–epithelial interactions, intestinal epithelium, Intestinal organoids, microbiome, probiotics",

author = "Jette Bornholdt and M{\"u}ller, {Christina V.} and Nielsen, {Maria Juul} and Jesper Strickertsson and Daria Rago and Yun Chen and Grzegorz Maciag and Jonathan Skov and Anja Wellejus and Schweiger, {Pawel J.} and Hansen, {Stine L.} and Christa Broholm and Ismail G{\"o}genur and Martti Maimets and Stine Sloth and Jakob Hendel and Adam Baker and Albin Sandelin and Jensen, {Kim B.}",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.",

year = "2023",

doi = "10.1080/19490976.2023.2281012",

language = "English",

volume = "15",

journal = "Gut Microbes",

issn = "1949-0976",

publisher = "Taylor & Francis",

number = "2",

}

RIS

TY - JOUR

T1 - Detecting host responses to microbial stimulation using primary epithelial organoids

AU - Bornholdt, Jette

AU - Müller, Christina V.

AU - Nielsen, Maria Juul

AU - Strickertsson, Jesper

AU - Rago, Daria

AU - Chen, Yun

AU - Maciag, Grzegorz

AU - Skov, Jonathan

AU - Wellejus, Anja

AU - Schweiger, Pawel J.

AU - Hansen, Stine L.

AU - Broholm, Christa

AU - Gögenur, Ismail

AU - Maimets, Martti

AU - Sloth, Stine

AU - Hendel, Jakob

AU - Baker, Adam

AU - Sandelin, Albin

AU - Jensen, Kim B.

PY - 2023

Y1 - 2023

N2 - The intestinal epithelium is constantly exposed to microbes residing in the lumen. Traditionally, the response to microbial interactions has been studied in cell lines derived from cancerous tissues, e.g. Caco-2. It is, however, unclear how the responses in these cancer cell lines reflect the responses of a normal epithelium and whether there might be microbial strain-specific effects. To address these questions, we derived organoids from the small intestine from a cohort of healthy individuals. Culturing intestinal epithelium on a flat laminin matrix induced their differentiation, facilitating analysis of microbial responses via the apical membrane normally exposed to the luminal content. Here, it was evident that the healthy epithelium across multiple individuals (n = 9) demonstrates robust acute both common and strain-specific responses to a range of probiotic bacterial strains (BB-12Ⓡ, LGGⓇ, DSM33361, and Bif195). Importantly, parallel experiments using the Caco-2 cell line provide no acute response. Collectively, we demonstrate that primary epithelial cells maintained as organoids represent a valuable resource for assessing interactions between the epithelium and luminal microbes across individuals, and that these models are likely to contribute to a better understanding of host microbe interactions.

AB - The intestinal epithelium is constantly exposed to microbes residing in the lumen. Traditionally, the response to microbial interactions has been studied in cell lines derived from cancerous tissues, e.g. Caco-2. It is, however, unclear how the responses in these cancer cell lines reflect the responses of a normal epithelium and whether there might be microbial strain-specific effects. To address these questions, we derived organoids from the small intestine from a cohort of healthy individuals. Culturing intestinal epithelium on a flat laminin matrix induced their differentiation, facilitating analysis of microbial responses via the apical membrane normally exposed to the luminal content. Here, it was evident that the healthy epithelium across multiple individuals (n = 9) demonstrates robust acute both common and strain-specific responses to a range of probiotic bacterial strains (BB-12Ⓡ, LGGⓇ, DSM33361, and Bif195). Importantly, parallel experiments using the Caco-2 cell line provide no acute response. Collectively, we demonstrate that primary epithelial cells maintained as organoids represent a valuable resource for assessing interactions between the epithelium and luminal microbes across individuals, and that these models are likely to contribute to a better understanding of host microbe interactions.

KW - bacterial–epithelial interactions

KW - intestinal epithelium

KW - Intestinal organoids

KW - microbiome

KW - probiotics

U2 - 10.1080/19490976.2023.2281012

DO - 10.1080/19490976.2023.2281012

M3 - Journal article

C2 - 37992398

AN - SCOPUS:85177660872

VL - 15

JO - Gut Microbes

JF - Gut Microbes

SN - 1949-0976

IS - 2

M1 - 2281012

ER -

ID: 375300973

Biologisk Institut