Dysregulation of Glucagon Secretion by Hyperglycemia-Induced Sodium-Dependent Reduction of ATP Production
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Dysregulation of Glucagon Secretion by Hyperglycemia-Induced Sodium-Dependent Reduction of ATP Production. / Knudsen, Jakob G.; Hamilton, Alexander; Ramracheya, Reshma; Tarasov, Andrei I.; Brereton, Melissa; Haythorne, Elizabeth; Chibalina, Margarita V.; Spégel, Peter; Mulder, Hindrik; Zhang, Quan; Ashcroft, Frances M.; Adam, Julie; Rorsman, Patrik.
I: Cell Metabolism, Bind 29, Nr. 2, 2019, s. 430-442, e1-e4.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Dysregulation of Glucagon Secretion by Hyperglycemia-Induced Sodium-Dependent Reduction of ATP Production
AU - Knudsen, Jakob G.
AU - Hamilton, Alexander
AU - Ramracheya, Reshma
AU - Tarasov, Andrei I.
AU - Brereton, Melissa
AU - Haythorne, Elizabeth
AU - Chibalina, Margarita V.
AU - Spégel, Peter
AU - Mulder, Hindrik
AU - Zhang, Quan
AU - Ashcroft, Frances M.
AU - Adam, Julie
AU - Rorsman, Patrik
N1 - Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Diabetes is a bihormonal disorder resulting from combined insulin and glucagon secretion defects. Mice lacking fumarase (Fh1) in their β cells (Fh1βKO mice) develop progressive hyperglycemia and dysregulated glucagon secretion similar to that seen in diabetic patients (too much at high glucose and too little at low glucose). The glucagon secretion defects are corrected by low concentrations of tolbutamide and prevented by the sodium-glucose transport (SGLT) inhibitor phlorizin. These data link hyperglycemia, intracellular Na+ accumulation, and acidification to impaired mitochondrial metabolism, reduced ATP production, and dysregulated glucagon secretion. Protein succination, reflecting reduced activity of fumarase, is observed in α cells from hyperglycemic Fh1βKO and β-V59M gain-of-function KATP channel mice, diabetic Goto-Kakizaki rats, and patients with type 2 diabetes. Succination is also observed in renal tubular cells and cardiomyocytes from hyperglycemic Fh1βKO mice, suggesting that the model can be extended to other SGLT-expressing cells and may explain part of the spectrum of diabetic complications.
AB - Diabetes is a bihormonal disorder resulting from combined insulin and glucagon secretion defects. Mice lacking fumarase (Fh1) in their β cells (Fh1βKO mice) develop progressive hyperglycemia and dysregulated glucagon secretion similar to that seen in diabetic patients (too much at high glucose and too little at low glucose). The glucagon secretion defects are corrected by low concentrations of tolbutamide and prevented by the sodium-glucose transport (SGLT) inhibitor phlorizin. These data link hyperglycemia, intracellular Na+ accumulation, and acidification to impaired mitochondrial metabolism, reduced ATP production, and dysregulated glucagon secretion. Protein succination, reflecting reduced activity of fumarase, is observed in α cells from hyperglycemic Fh1βKO and β-V59M gain-of-function KATP channel mice, diabetic Goto-Kakizaki rats, and patients with type 2 diabetes. Succination is also observed in renal tubular cells and cardiomyocytes from hyperglycemic Fh1βKO mice, suggesting that the model can be extended to other SGLT-expressing cells and may explain part of the spectrum of diabetic complications.
U2 - 10.1016/j.cmet.2018.10.003
DO - 10.1016/j.cmet.2018.10.003
M3 - Journal article
C2 - 30415925
VL - 29
SP - 430-442, e1-e4
JO - Cell Metabolism
JF - Cell Metabolism
SN - 1550-4131
IS - 2
ER -
ID: 220850613