Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells
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Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells. / Maar, T E; Lund, Trine Meldgaard; Gegelashvili, G; Hartmann-Petersen, R; Moran, Michael J.; Pasantes-Morales, H; Berezin, V; Bock, E; Schousboe, A.
I: Amino Acids, Bind 15, Nr. 1, 1998, s. 77-88.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells
AU - Maar, T E
AU - Lund, Trine Meldgaard
AU - Gegelashvili, G
AU - Hartmann-Petersen, R
AU - Moran, Michael J.
AU - Pasantes-Morales, H
AU - Berezin, V
AU - Bock, E
AU - Schousboe, A
PY - 1998
Y1 - 1998
N2 - Cultures of dissociated cerebellum from 5- to 6-day-old mice as well as of the N2A neuronal cell line were exposed to guanidino ethane sulfonate (GES, 2-5 mM) to reduce the cellular taurine content. Control cultures were kept in culture medium or medium containing 2-5 mM GES plus 2-5 mM taurine to restore the intracellular taurine content. Taurine depletion led to changes in the expression of certain splice variants of NCAM mRNA such as the AAG and the VASE containing forms, while no differences were seen in the expression of the three forms of NCAM protein. In the N2A cells taurine depletion led to a decreased migration rate of the cells. The results suggest that the reduced migration rate of neurons caused by taurine depletion may be correlated to changes in expression of certain adhesion molecules such as NCAM. Moreover, taurine appears to be involved in regulation of transcription processes.
AB - Cultures of dissociated cerebellum from 5- to 6-day-old mice as well as of the N2A neuronal cell line were exposed to guanidino ethane sulfonate (GES, 2-5 mM) to reduce the cellular taurine content. Control cultures were kept in culture medium or medium containing 2-5 mM GES plus 2-5 mM taurine to restore the intracellular taurine content. Taurine depletion led to changes in the expression of certain splice variants of NCAM mRNA such as the AAG and the VASE containing forms, while no differences were seen in the expression of the three forms of NCAM protein. In the N2A cells taurine depletion led to a decreased migration rate of the cells. The results suggest that the reduced migration rate of neurons caused by taurine depletion may be correlated to changes in expression of certain adhesion molecules such as NCAM. Moreover, taurine appears to be involved in regulation of transcription processes.
KW - Alternative Splicing
KW - Animals
KW - Biological Transport
KW - Cell Movement
KW - Cells, Cultured
KW - Cerebellum
KW - Gene Expression
KW - Mice
KW - Nerve Net
KW - Neural Cell Adhesion Molecules
KW - Neurons
KW - RNA, Messenger
KW - Taurine
U2 - 10.1007/BF01345281
DO - 10.1007/BF01345281
M3 - Journal article
C2 - 9871488
VL - 15
SP - 77
EP - 88
JO - Amino Acids
JF - Amino Acids
SN - 0939-4451
IS - 1
ER -
ID: 206222