Familial relationships in hyperthermo- and acidophilic archaeal viruses
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Familial relationships in hyperthermo- and acidophilic archaeal viruses. / Happonen, Lotta Johanna; Redder, Peter; Peng, Xu; Reigstad, Laila Johanne; Prangishvili, David; Butcher, Sarah Jane.
I: Journal of Virology, Bind 84, Nr. 9, 01.05.2010, s. 4747-54.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Familial relationships in hyperthermo- and acidophilic archaeal viruses
AU - Happonen, Lotta Johanna
AU - Redder, Peter
AU - Peng, Xu
AU - Reigstad, Laila Johanne
AU - Prangishvili, David
AU - Butcher, Sarah Jane
PY - 2010/5/1
Y1 - 2010/5/1
N2 - Archaea often live in extreme, harsh environments such as acidic hot springs and hypersaline waters. To date, only two icosahedrally symmetric, membrane-containing archaeal viruses, SH1 and Sulfolobus turreted icosahedral virus (STIV), have been described in detail. We report the sequence and three-dimensional structure of a third such virus isolated from a hyperthermoacidophilic crenarchaeon, Sulfolobus strain G4ST-2. Characterization of this new isolate revealed it to be similar to STIV on the levels of genome and structural organization. The genome organization indicates that these two viruses have diverged from a common ancestor. Interestingly, the prominent surface turrets of the two viruses are strikingly different. By sequencing and mass spectrometry, we mapped several large insertions and deletions in the known structural proteins that could account for these differences and showed that both viruses can infect the same host. A combination of genomic and proteomic analyses revealed important new insights into the structural organization of these viruses and added to our limited knowledge of archaeal virus life cycles and host-cell interactions.
AB - Archaea often live in extreme, harsh environments such as acidic hot springs and hypersaline waters. To date, only two icosahedrally symmetric, membrane-containing archaeal viruses, SH1 and Sulfolobus turreted icosahedral virus (STIV), have been described in detail. We report the sequence and three-dimensional structure of a third such virus isolated from a hyperthermoacidophilic crenarchaeon, Sulfolobus strain G4ST-2. Characterization of this new isolate revealed it to be similar to STIV on the levels of genome and structural organization. The genome organization indicates that these two viruses have diverged from a common ancestor. Interestingly, the prominent surface turrets of the two viruses are strikingly different. By sequencing and mass spectrometry, we mapped several large insertions and deletions in the known structural proteins that could account for these differences and showed that both viruses can infect the same host. A combination of genomic and proteomic analyses revealed important new insights into the structural organization of these viruses and added to our limited knowledge of archaeal virus life cycles and host-cell interactions.
KW - Archaeal Viruses
KW - Cluster Analysis
KW - DNA, Archaeal
KW - DNA, Ribosomal
KW - DNA, Viral
KW - Gene Order
KW - Genome, Viral
KW - Microscopy, Electron, Transmission
KW - Models, Biological
KW - Models, Molecular
KW - Molecular Sequence Data
KW - Proteome
KW - RNA, Ribosomal, 16S
KW - Sequence Analysis, DNA
KW - Sequence Homology
KW - Sulfolobus
KW - Synteny
KW - Viral Proteins
KW - Virion
U2 - 10.1128/JVI.02156-09
DO - 10.1128/JVI.02156-09
M3 - Journal article
C2 - 20164227
VL - 84
SP - 4747
EP - 4754
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 9
ER -
ID: 33968663