Gene regulation in response to protein disulphide isomerase deficiency
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Gene regulation in response to protein disulphide isomerase deficiency. / Nørgaard, Per; Tachibana, Christine; Bruun, Anette W; Winther, Jakob R.
I: Yeast, Bind 20, Nr. 7, 2003, s. 645-652.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Gene regulation in response to protein disulphide isomerase deficiency
AU - Nørgaard, Per
AU - Tachibana, Christine
AU - Bruun, Anette W
AU - Winther, Jakob R.
N1 - Copyright 2003 John Wiley & Sons, Ltd.
PY - 2003
Y1 - 2003
N2 - We have examined the activities of promoters of a number of yeast genes encoding resident endoplasmic reticulum proteins, and found increased expression in a strain with severe protein disulphide isomerase deficiency. Serial deletion in the promoter of the MPD1 gene, which encodes a PDI1-homologue, revealed a cis-acting element responding to deficiency of protein disulphide isomerase activity (designated CERP). The presence of the sequence element is necessary and sufficient for the upregulation in response to disulphide isomerase deficiency, as measured by a minimal promoter containing the CERP element. The sequence (GACACG) does not resemble the unfolded protein response element. It is present in the upstream regions of the MPD1, MPD2, KAR2, PDI1 and ERO1 genes.
AB - We have examined the activities of promoters of a number of yeast genes encoding resident endoplasmic reticulum proteins, and found increased expression in a strain with severe protein disulphide isomerase deficiency. Serial deletion in the promoter of the MPD1 gene, which encodes a PDI1-homologue, revealed a cis-acting element responding to deficiency of protein disulphide isomerase activity (designated CERP). The presence of the sequence element is necessary and sufficient for the upregulation in response to disulphide isomerase deficiency, as measured by a minimal promoter containing the CERP element. The sequence (GACACG) does not resemble the unfolded protein response element. It is present in the upstream regions of the MPD1, MPD2, KAR2, PDI1 and ERO1 genes.
KW - Base Sequence
KW - DNA, Fungal
KW - Endoplasmic Reticulum
KW - Gene Expression Regulation, Fungal
KW - Genes, Fungal
KW - Isomerases
KW - Promoter Regions, Genetic
KW - Protein Disulfide-Isomerases
KW - Repressor Proteins
KW - Saccharomyces cerevisiae
KW - Saccharomyces cerevisiae Proteins
KW - Sequence Deletion
U2 - 10.1002/yea.978
DO - 10.1002/yea.978
M3 - Journal article
C2 - 12734802
VL - 20
SP - 645
EP - 652
JO - Yeast
JF - Yeast
SN - 0749-503X
IS - 7
ER -
ID: 43973624