Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.
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Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae. / López-Mirabal, H Reynaldo; Winther, Jakob R; Kielland-Brandt, Morten C.
I: Bioscience, Biotechnology, and Biochemistry, Bind 71, Nr. 12, 2007, s. 2934-42.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.
AU - López-Mirabal, H Reynaldo
AU - Winther, Jakob R
AU - Kielland-Brandt, Morten C
PY - 2007
Y1 - 2007
N2 - The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Udgivelsesdato: 2007-Dec
AB - The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Udgivelsesdato: 2007-Dec
M3 - Journal article
C2 - 18071269
VL - 71
SP - 2934
EP - 2942
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 12
ER -
ID: 3090399