Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae. / López-Mirabal, H Reynaldo; Winther, Jakob R; Kielland-Brandt, Morten C.

I: Bioscience, Biotechnology, and Biochemistry, Bind 71, Nr. 12, 2007, s. 2934-42.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

López-Mirabal, HR, Winther, JR & Kielland-Brandt, MC 2007, 'Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.', Bioscience, Biotechnology, and Biochemistry, bind 71, nr. 12, s. 2934-42.

APA

López-Mirabal, H. R., Winther, J. R., & Kielland-Brandt, M. C. (2007). Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae. Bioscience, Biotechnology, and Biochemistry, 71(12), 2934-42.

Vancouver

López-Mirabal HR, Winther JR, Kielland-Brandt MC. Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae. Bioscience, Biotechnology, and Biochemistry. 2007;71(12):2934-42.

Author

López-Mirabal, H Reynaldo ; Winther, Jakob R ; Kielland-Brandt, Morten C. / Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae. I: Bioscience, Biotechnology, and Biochemistry. 2007 ; Bind 71, Nr. 12. s. 2934-42.

Bibtex

@article{d7ab59c0eeb311dcbee902004c4f4f50,
title = "Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.",
abstract = "The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Udgivelsesdato: 2007-Dec",
author = "L{\'o}pez-Mirabal, {H Reynaldo} and Winther, {Jakob R} and Kielland-Brandt, {Morten C}",
year = "2007",
language = "English",
volume = "71",
pages = "2934--42",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Taylor & Francis",
number = "12",

}

RIS

TY - JOUR

T1 - Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.

AU - López-Mirabal, H Reynaldo

AU - Winther, Jakob R

AU - Kielland-Brandt, Morten C

PY - 2007

Y1 - 2007

N2 - The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Udgivelsesdato: 2007-Dec

AB - The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Udgivelsesdato: 2007-Dec

M3 - Journal article

C2 - 18071269

VL - 71

SP - 2934

EP - 2942

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 12

ER -

ID: 3090399