Genome editing in archaeal viruses and endogenous viral protein purification

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Standard

Genome editing in archaeal viruses and endogenous viral protein purification. / Alfastsen, Lauge; Peng, Xu; Bhoobalan-Chitty, Yuvaraj.

I: STAR Protocols, Bind 2, Nr. 4, 100791, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Alfastsen, L, Peng, X & Bhoobalan-Chitty, Y 2021, 'Genome editing in archaeal viruses and endogenous viral protein purification', STAR Protocols, bind 2, nr. 4, 100791. https://doi.org/10.1016/j.xpro.2021.100791

APA

Alfastsen, L., Peng, X., & Bhoobalan-Chitty, Y. (2021). Genome editing in archaeal viruses and endogenous viral protein purification. STAR Protocols, 2(4), [100791]. https://doi.org/10.1016/j.xpro.2021.100791

Vancouver

Alfastsen L, Peng X, Bhoobalan-Chitty Y. Genome editing in archaeal viruses and endogenous viral protein purification. STAR Protocols. 2021;2(4). 100791. https://doi.org/10.1016/j.xpro.2021.100791

Author

Alfastsen, Lauge ; Peng, Xu ; Bhoobalan-Chitty, Yuvaraj. / Genome editing in archaeal viruses and endogenous viral protein purification. I: STAR Protocols. 2021 ; Bind 2, Nr. 4.

Bibtex

@article{8e5fd66d89284d7780591c0925367652,
title = "Genome editing in archaeal viruses and endogenous viral protein purification",
abstract = "Archaea-infecting viruses are morphologically and genomically among the most diverse entities. Unfortunately, they are also fairly understudied due to a lack of efficient genetic tools. Here, we present a detailed protocol for the CRISPR/Cas-based genome editing of the virus SIRV2 infecting the genus Sulfolobus, which could easily be adapted to other archaeal viruses. This protocol also includes the procedure for endogenous viral protein purification and identification, allowing for assessing the molecular mechanisms behind virus life cycle and virus-host interactions. For complete details on the use and execution of this protocol, please refer to Mayo-Mu{\~n}oz et al. (2018) and Bhoobalan-Chitty et al. (2019).",
keywords = "CRISPR, Genetics, Microbiology, Molecular Biology, Protein Biochemistry, Protein expression and purification",
author = "Lauge Alfastsen and Xu Peng and Yuvaraj Bhoobalan-Chitty",
note = "Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
doi = "10.1016/j.xpro.2021.100791",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Genome editing in archaeal viruses and endogenous viral protein purification

AU - Alfastsen, Lauge

AU - Peng, Xu

AU - Bhoobalan-Chitty, Yuvaraj

N1 - Publisher Copyright: © 2021 The Authors

PY - 2021

Y1 - 2021

N2 - Archaea-infecting viruses are morphologically and genomically among the most diverse entities. Unfortunately, they are also fairly understudied due to a lack of efficient genetic tools. Here, we present a detailed protocol for the CRISPR/Cas-based genome editing of the virus SIRV2 infecting the genus Sulfolobus, which could easily be adapted to other archaeal viruses. This protocol also includes the procedure for endogenous viral protein purification and identification, allowing for assessing the molecular mechanisms behind virus life cycle and virus-host interactions. For complete details on the use and execution of this protocol, please refer to Mayo-Muñoz et al. (2018) and Bhoobalan-Chitty et al. (2019).

AB - Archaea-infecting viruses are morphologically and genomically among the most diverse entities. Unfortunately, they are also fairly understudied due to a lack of efficient genetic tools. Here, we present a detailed protocol for the CRISPR/Cas-based genome editing of the virus SIRV2 infecting the genus Sulfolobus, which could easily be adapted to other archaeal viruses. This protocol also includes the procedure for endogenous viral protein purification and identification, allowing for assessing the molecular mechanisms behind virus life cycle and virus-host interactions. For complete details on the use and execution of this protocol, please refer to Mayo-Muñoz et al. (2018) and Bhoobalan-Chitty et al. (2019).

KW - CRISPR

KW - Genetics

KW - Microbiology

KW - Molecular Biology

KW - Protein Biochemistry

KW - Protein expression and purification

U2 - 10.1016/j.xpro.2021.100791

DO - 10.1016/j.xpro.2021.100791

M3 - Journal article

C2 - 34585154

AN - SCOPUS:85122827532

VL - 2

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 4

M1 - 100791

ER -

ID: 291362159