Genome-wide occupancy profile of mediator and the Srb8-11 module reveals interactions with coding regions
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Genome-wide occupancy profile of mediator and the Srb8-11 module reveals interactions with coding regions. / Zhu, Xuefeng; Wirén, Marianna; Sinha, Indranil; Rasmussen, Nina N.; Linder, Tomas; Holmberg, Steen; Ekwall, Karl; Gustafsson, Claes M.
I: Molecular Cell, Bind 22, Nr. 2, 2006, s. 169-78.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Genome-wide occupancy profile of mediator and the Srb8-11 module reveals interactions with coding regions
AU - Zhu, Xuefeng
AU - Wirén, Marianna
AU - Sinha, Indranil
AU - Rasmussen, Nina N.
AU - Linder, Tomas
AU - Holmberg, Steen
AU - Ekwall, Karl
AU - Gustafsson, Claes M
N1 - Keywords: Alleles; Chromatin Immunoprecipitation; DNA, Fungal; DNA, Intergenic; Down-Regulation; Gene Expression Profiling; Genes, Fungal; Genome, Fungal; Nucleic Acid Amplification Techniques; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Promoter Regions, Genetic; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Transcription, Genetic
PY - 2006
Y1 - 2006
N2 - Mediator exists in a free form containing the Med12, Med13, CDK8, and CycC subunits (the Srb8-11 module) and a smaller form, which lacks these four subunits and associates with RNA polymerase II (Pol II), forming a holoenzyme. We use chromatin immunoprecipitation (ChIP) and DNA microarrays to investigate genome-wide localization of Mediator and the Srb8-11 module in fission yeast. Mediator and the Srb8-11 module display similar binding patterns, and interactions with promoters and upstream activating sequences correlate with increased transcription activity. Unexpectedly, Mediator also interacts with the downstream coding region of many genes. These interactions display a negative bias for positions closer to the 5' ends of open reading frames (ORFs) and appear functionally important, because downregulation of transcription in a temperature-sensitive med17 mutant strain correlates with increased Mediator occupancy in the coding region. We propose that Mediator coordinates transcription initiation with transcriptional events in the coding region of eukaryotic genes.
AB - Mediator exists in a free form containing the Med12, Med13, CDK8, and CycC subunits (the Srb8-11 module) and a smaller form, which lacks these four subunits and associates with RNA polymerase II (Pol II), forming a holoenzyme. We use chromatin immunoprecipitation (ChIP) and DNA microarrays to investigate genome-wide localization of Mediator and the Srb8-11 module in fission yeast. Mediator and the Srb8-11 module display similar binding patterns, and interactions with promoters and upstream activating sequences correlate with increased transcription activity. Unexpectedly, Mediator also interacts with the downstream coding region of many genes. These interactions display a negative bias for positions closer to the 5' ends of open reading frames (ORFs) and appear functionally important, because downregulation of transcription in a temperature-sensitive med17 mutant strain correlates with increased Mediator occupancy in the coding region. We propose that Mediator coordinates transcription initiation with transcriptional events in the coding region of eukaryotic genes.
U2 - 10.1016/j.molcel.2006.03.032
DO - 10.1016/j.molcel.2006.03.032
M3 - Journal article
C2 - 16630887
VL - 22
SP - 169
EP - 178
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 2
ER -
ID: 9250969