High-throughput siRNA screening applied to the ubiquitin-proteasome system

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Standard

High-throughput siRNA screening applied to the ubiquitin-proteasome system. / Poulsen, Esben Guldahl; Nielsen, Sofie V.; Pietras, Elin J.; Johansen, Jens Vilstrup; Steinhauer, Cornelia; Hartmann-Petersen, Rasmus.

Proteostasis. red. / Rune Matthiesen. Springer, 2016. s. 421-439 (Methods in Molecular Biology, Bind 1449).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Poulsen, EG, Nielsen, SV, Pietras, EJ, Johansen, JV, Steinhauer, C & Hartmann-Petersen, R 2016, High-throughput siRNA screening applied to the ubiquitin-proteasome system. i R Matthiesen (red.), Proteostasis. Springer, Methods in Molecular Biology, bind 1449, s. 421-439. https://doi.org/10.1007/978-1-4939-3756-1_28

APA

Poulsen, E. G., Nielsen, S. V., Pietras, E. J., Johansen, J. V., Steinhauer, C., & Hartmann-Petersen, R. (2016). High-throughput siRNA screening applied to the ubiquitin-proteasome system. I R. Matthiesen (red.), Proteostasis (s. 421-439). Springer. Methods in Molecular Biology Bind 1449 https://doi.org/10.1007/978-1-4939-3756-1_28

Vancouver

Poulsen EG, Nielsen SV, Pietras EJ, Johansen JV, Steinhauer C, Hartmann-Petersen R. High-throughput siRNA screening applied to the ubiquitin-proteasome system. I Matthiesen R, red., Proteostasis. Springer. 2016. s. 421-439. (Methods in Molecular Biology, Bind 1449). https://doi.org/10.1007/978-1-4939-3756-1_28

Author

Poulsen, Esben Guldahl ; Nielsen, Sofie V. ; Pietras, Elin J. ; Johansen, Jens Vilstrup ; Steinhauer, Cornelia ; Hartmann-Petersen, Rasmus. / High-throughput siRNA screening applied to the ubiquitin-proteasome system. Proteostasis. red. / Rune Matthiesen. Springer, 2016. s. 421-439 (Methods in Molecular Biology, Bind 1449).

Bibtex

@inbook{9ac3419bd5da4841aa0532d150e8e24e,
title = "High-throughput siRNA screening applied to the ubiquitin-proteasome system",
abstract = "The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function.",
keywords = "Journal Article",
author = "Poulsen, {Esben Guldahl} and Nielsen, {Sofie V.} and Pietras, {Elin J.} and Johansen, {Jens Vilstrup} and Cornelia Steinhauer and Rasmus Hartmann-Petersen",
year = "2016",
doi = "10.1007/978-1-4939-3756-1_28",
language = "English",
isbn = "978-1-4939-3754-7",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "421--439",
editor = "Rune Matthiesen",
booktitle = "Proteostasis",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - High-throughput siRNA screening applied to the ubiquitin-proteasome system

AU - Poulsen, Esben Guldahl

AU - Nielsen, Sofie V.

AU - Pietras, Elin J.

AU - Johansen, Jens Vilstrup

AU - Steinhauer, Cornelia

AU - Hartmann-Petersen, Rasmus

PY - 2016

Y1 - 2016

N2 - The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function.

AB - The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function.

KW - Journal Article

U2 - 10.1007/978-1-4939-3756-1_28

DO - 10.1007/978-1-4939-3756-1_28

M3 - Book chapter

C2 - 27613054

SN - 978-1-4939-3754-7

T3 - Methods in Molecular Biology

SP - 421

EP - 439

BT - Proteostasis

A2 - Matthiesen, Rune

PB - Springer

ER -

ID: 179133797