How phosphorylation impacts intrinsically disordered proteins and their function
Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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How phosphorylation impacts intrinsically disordered proteins and their function. / Newcombe, Estella A.; Delaforge, Elise; Hartmann-Petersen, Rasmus; Skriver, Karen; Kragelund, Birthe B.
I: Essays in Biochemistry, Bind 66, Nr. 7, 2022, s. 901-913.Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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TY - JOUR
T1 - How phosphorylation impacts intrinsically disordered proteins and their function
AU - Newcombe, Estella A.
AU - Delaforge, Elise
AU - Hartmann-Petersen, Rasmus
AU - Skriver, Karen
AU - Kragelund, Birthe B.
N1 - Publisher Copyright: © 2022 The Author(s).
PY - 2022
Y1 - 2022
N2 - Phosphorylation is the most common post-translational modification (PTM) in eukaryotes, occurring particularly frequently in intrinsically disordered proteins (IDPs). These proteins are highly flexible and dynamic by nature. Thus, it is intriguing that the addition of a single phosphoryl group to a disordered chain can impact its function so dramatically. Furthermore, as many IDPs carry multiple phosphorylation sites, the number of possible states increases, enabling larger complexities and novel mechanisms. Although a chemically simple and well-understood process, the impact of phosphorylation on the conformational ensemble and molecular function of IDPs, not to mention biological output, is highly complex and diverse. Since the discovery of the first phosphorylation site in proteins 75 years ago, we have come to a much better understanding of how this PTM works, but with the diversity of IDPs and their capacity for carrying multiple phosphoryl groups, the complexity grows. In this Essay, we highlight some of the basic effects of IDP phosphorylation, allowing it to serve as starting point when embarking on studies into this topic. We further describe how recent complex cases of multisite phosphorylation of IDPs have been instrumental in widening our view on the effect of protein phosphorylation. Finally, we put forward perspectives on the phosphorylation of IDPs, both in relation to disease and in context of other PTMs; areas where deep insight remains to be uncovered.
AB - Phosphorylation is the most common post-translational modification (PTM) in eukaryotes, occurring particularly frequently in intrinsically disordered proteins (IDPs). These proteins are highly flexible and dynamic by nature. Thus, it is intriguing that the addition of a single phosphoryl group to a disordered chain can impact its function so dramatically. Furthermore, as many IDPs carry multiple phosphorylation sites, the number of possible states increases, enabling larger complexities and novel mechanisms. Although a chemically simple and well-understood process, the impact of phosphorylation on the conformational ensemble and molecular function of IDPs, not to mention biological output, is highly complex and diverse. Since the discovery of the first phosphorylation site in proteins 75 years ago, we have come to a much better understanding of how this PTM works, but with the diversity of IDPs and their capacity for carrying multiple phosphoryl groups, the complexity grows. In this Essay, we highlight some of the basic effects of IDP phosphorylation, allowing it to serve as starting point when embarking on studies into this topic. We further describe how recent complex cases of multisite phosphorylation of IDPs have been instrumental in widening our view on the effect of protein phosphorylation. Finally, we put forward perspectives on the phosphorylation of IDPs, both in relation to disease and in context of other PTMs; areas where deep insight remains to be uncovered.
KW - allosteric regulation
KW - intrinsically disordered proteins
KW - molecular mechanisms
KW - phosphorylation/dephosphorylation
KW - post translational modification
KW - protein-protein interactions
U2 - 10.1042/EBC20220060
DO - 10.1042/EBC20220060
M3 - Review
C2 - 36350035
AN - SCOPUS:85144586127
VL - 66
SP - 901
EP - 913
JO - Essays in Biochemistry
JF - Essays in Biochemistry
SN - 0071-1365
IS - 7
ER -
ID: 330886825