Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)

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Standard

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA). / Schicketanz, Muriel Leandra; Długosz, Paulina; Zhang, Yong Everett.

I: Journal of visualized experiments : JoVE, Bind 169, e62331, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Schicketanz, ML, Długosz, P & Zhang, YE 2021, 'Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)', Journal of visualized experiments : JoVE, bind 169, e62331. https://doi.org/10.3791/62331

APA

Schicketanz, M. L., Długosz, P., & Zhang, Y. E. (2021). Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA). Journal of visualized experiments : JoVE, 169, [e62331]. https://doi.org/10.3791/62331

Vancouver

Schicketanz ML, Długosz P, Zhang YE. Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA). Journal of visualized experiments : JoVE. 2021;169. e62331. https://doi.org/10.3791/62331

Author

Schicketanz, Muriel Leandra ; Długosz, Paulina ; Zhang, Yong Everett. / Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA). I: Journal of visualized experiments : JoVE. 2021 ; Bind 169.

Bibtex

@article{ce8a8dedcdd94412bb0aa8da71ababf9,

title = "Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)",

abstract = "The past decade has seen tremendous progress in the understanding of small signaling molecules in bacterial physiology. In particular, the target proteins of several nucleotide-derived secondary messengers (NSMs) have been systematically identified and studied in model organisms. These achievements are mainly due to the development of several new techniques including the capture compound technique and the differential radial capillary action of ligand assay (DRaCALA), which were used to systematically identify target proteins of these small molecules. This paper describes the use of the NSMs, guanosine penta-and tetraphosphates (p)ppGpp, as an example and video demonstration of the DRaCALA technique. Using DRaCALA, 9 out of 20 known and 12 new target proteins of (p)ppGpp were identified in the model organism, Escherichia coli K-12, demonstrating the power of this assay. In principle, DRaCALA could be used for studying small ligands that can be labeled by radioactive isotopes or fluorescent dyes. The critical steps, pros, and cons of DRaCALA are discussed here for further application of this technique.",

author = "Schicketanz, {Muriel Leandra} and Paulina D{\l}ugosz and Zhang, {Yong Everett}",

note = "Publisher Copyright: {\textcopyright} 2021 JoVE Journal of Visualized Experiments.",

year = "2021",

doi = "10.3791/62331",

language = "English",

volume = "169",

journal = "Journal of Visualized Experiments",

issn = "1940-087X",

publisher = "Journal of Visualized Experiments",

}

RIS

TY - JOUR

T1 - Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)

AU - Schicketanz, Muriel Leandra

AU - Długosz, Paulina

AU - Zhang, Yong Everett

PY - 2021

Y1 - 2021

N2 - The past decade has seen tremendous progress in the understanding of small signaling molecules in bacterial physiology. In particular, the target proteins of several nucleotide-derived secondary messengers (NSMs) have been systematically identified and studied in model organisms. These achievements are mainly due to the development of several new techniques including the capture compound technique and the differential radial capillary action of ligand assay (DRaCALA), which were used to systematically identify target proteins of these small molecules. This paper describes the use of the NSMs, guanosine penta-and tetraphosphates (p)ppGpp, as an example and video demonstration of the DRaCALA technique. Using DRaCALA, 9 out of 20 known and 12 new target proteins of (p)ppGpp were identified in the model organism, Escherichia coli K-12, demonstrating the power of this assay. In principle, DRaCALA could be used for studying small ligands that can be labeled by radioactive isotopes or fluorescent dyes. The critical steps, pros, and cons of DRaCALA are discussed here for further application of this technique.

AB - The past decade has seen tremendous progress in the understanding of small signaling molecules in bacterial physiology. In particular, the target proteins of several nucleotide-derived secondary messengers (NSMs) have been systematically identified and studied in model organisms. These achievements are mainly due to the development of several new techniques including the capture compound technique and the differential radial capillary action of ligand assay (DRaCALA), which were used to systematically identify target proteins of these small molecules. This paper describes the use of the NSMs, guanosine penta-and tetraphosphates (p)ppGpp, as an example and video demonstration of the DRaCALA technique. Using DRaCALA, 9 out of 20 known and 12 new target proteins of (p)ppGpp were identified in the model organism, Escherichia coli K-12, demonstrating the power of this assay. In principle, DRaCALA could be used for studying small ligands that can be labeled by radioactive isotopes or fluorescent dyes. The critical steps, pros, and cons of DRaCALA are discussed here for further application of this technique.

U2 - 10.3791/62331

DO - 10.3791/62331

M3 - Journal article

C2 - 33818559

AN - SCOPUS:85103922047

VL - 169

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

M1 - e62331

ER -

ID: 262892574

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