Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions

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Standard

Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions. / Awan, Aashir; Oliveri, Roberto S; Jensen, Pernille L; Christensen, Søren T; Andersen, Claus Yding.

I: Methods in Molecular Biology, Bind 584, 01.01.2010, s. 195-210.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Awan, A, Oliveri, RS, Jensen, PL, Christensen, ST & Andersen, CY 2010, 'Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions', Methods in Molecular Biology, bind 584, s. 195-210. https://doi.org/10.1007/978-1-60761-369-5_11

APA

Awan, A., Oliveri, R. S., Jensen, P. L., Christensen, S. T., & Andersen, C. Y. (2010). Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions. Methods in Molecular Biology, 584, 195-210. https://doi.org/10.1007/978-1-60761-369-5_11

Vancouver

Awan A, Oliveri RS, Jensen PL, Christensen ST, Andersen CY. Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions. Methods in Molecular Biology. 2010 jan. 1;584:195-210. https://doi.org/10.1007/978-1-60761-369-5_11

Author

Awan, Aashir ; Oliveri, Roberto S ; Jensen, Pernille L ; Christensen, Søren T ; Andersen, Claus Yding. / Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions. I: Methods in Molecular Biology. 2010 ; Bind 584. s. 195-210.

Bibtex

@article{1cba8fe6fbc74272b34c7a0992718232,
title = "Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions",
abstract = "This chapter describes the procedures in order to do immunofluorescence (IF) microscopy and quantitative PCR (qPCR) analysis of human embryonic stem cells (hESCs) grown specifically under feeder-free conditions. A detailed protocol outlining the steps from initially growing the cells, passaging onto 16-well glass chambers, and continuing with the general IF and qPCR steps will be provided. The techniques will be illustrated with new results on cellular localization of transcriptional factors and components of the Hedgehog, Wnt, and PDGF signaling pathways to primary cilia in stem cell maintenance and differentiation. Furthermore, a sample qPCR experiment will be shown illustrating that these techniques can be important tools in answering basic questions about hESC biology.",
keywords = "Base Sequence, Biological Markers, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cilia, DNA Primers, Embryonic Stem Cells, Genetic Markers, Humans, Microscopy, Fluorescence, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction",
author = "Aashir Awan and Oliveri, {Roberto S} and Jensen, {Pernille L} and Christensen, {S{\o}ren T} and Andersen, {Claus Yding}",
year = "2010",
month = jan,
day = "1",
doi = "10.1007/978-1-60761-369-5_11",
language = "English",
volume = "584",
pages = "195--210",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions

AU - Awan, Aashir

AU - Oliveri, Roberto S

AU - Jensen, Pernille L

AU - Christensen, Søren T

AU - Andersen, Claus Yding

PY - 2010/1/1

Y1 - 2010/1/1

N2 - This chapter describes the procedures in order to do immunofluorescence (IF) microscopy and quantitative PCR (qPCR) analysis of human embryonic stem cells (hESCs) grown specifically under feeder-free conditions. A detailed protocol outlining the steps from initially growing the cells, passaging onto 16-well glass chambers, and continuing with the general IF and qPCR steps will be provided. The techniques will be illustrated with new results on cellular localization of transcriptional factors and components of the Hedgehog, Wnt, and PDGF signaling pathways to primary cilia in stem cell maintenance and differentiation. Furthermore, a sample qPCR experiment will be shown illustrating that these techniques can be important tools in answering basic questions about hESC biology.

AB - This chapter describes the procedures in order to do immunofluorescence (IF) microscopy and quantitative PCR (qPCR) analysis of human embryonic stem cells (hESCs) grown specifically under feeder-free conditions. A detailed protocol outlining the steps from initially growing the cells, passaging onto 16-well glass chambers, and continuing with the general IF and qPCR steps will be provided. The techniques will be illustrated with new results on cellular localization of transcriptional factors and components of the Hedgehog, Wnt, and PDGF signaling pathways to primary cilia in stem cell maintenance and differentiation. Furthermore, a sample qPCR experiment will be shown illustrating that these techniques can be important tools in answering basic questions about hESC biology.

KW - Base Sequence

KW - Biological Markers

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Cell Proliferation

KW - Cilia

KW - DNA Primers

KW - Embryonic Stem Cells

KW - Genetic Markers

KW - Humans

KW - Microscopy, Fluorescence

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Signal Transduction

U2 - 10.1007/978-1-60761-369-5_11

DO - 10.1007/978-1-60761-369-5_11

M3 - Journal article

C2 - 19907979

VL - 584

SP - 195

EP - 210

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 33226211