Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Standard

Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways. / Vestergaard, Maj Linea; Awan, Aashir; Warzecha, Caroline Becker; Christensen, Søren Tvorup; Andersen, Claus Yding.

Human Embryonic Stem Cell Protocols. Bind 1307 Springer Science+Business Media, 2016. s. 123-140 (Methods in molecular biology (Clifton, N.J.)).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Vestergaard, ML, Awan, A, Warzecha, CB, Christensen, ST & Andersen, CY 2016, Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways. i Human Embryonic Stem Cell Protocols. bind 1307, Springer Science+Business Media, Methods in molecular biology (Clifton, N.J.), s. 123-140. https://doi.org/10.1007/7651_2014_127

APA

Vestergaard, M. L., Awan, A., Warzecha, C. B., Christensen, S. T., & Andersen, C. Y. (2016). Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways. I Human Embryonic Stem Cell Protocols (Bind 1307, s. 123-140). Springer Science+Business Media. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/7651_2014_127

Vancouver

Vestergaard ML, Awan A, Warzecha CB, Christensen ST, Andersen CY. Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways. I Human Embryonic Stem Cell Protocols. Bind 1307. Springer Science+Business Media. 2016. s. 123-140. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/7651_2014_127

Author

Vestergaard, Maj Linea ; Awan, Aashir ; Warzecha, Caroline Becker ; Christensen, Søren Tvorup ; Andersen, Claus Yding. / Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways. Human Embryonic Stem Cell Protocols. Bind 1307 Springer Science+Business Media, 2016. s. 123-140 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{1730d00167114e6daf9eec101b62a8cb,
title = "Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways",
abstract = "This chapter describes the procedures for immunofluorescence microscopy (IFM) and quantitative PCR (qPCR) analyses of human embryonic stem cells (hESCs) grown specifically under feeder-free conditions. A detailed protocol is provided outlining the steps from initially growing the cells, passaging onto 16-well glass chambers, and continuing with the general IFM and qPCR anlysis. The techniques are illustrated with results on cellular localization of transcriptional factors and components of the Hedgehog, Wnt, PDGF, and TGFβ signaling pathways to primary cilia in stem cell maintenance and differentiation. Furthermore, a sample qPCR experiment is experimentally shown illustrating that these techniques can be important tools in answering basic questions about hESC biology.",
author = "Vestergaard, {Maj Linea} and Aashir Awan and Warzecha, {Caroline Becker} and Christensen, {S{\o}ren Tvorup} and Andersen, {Claus Yding}",
year = "2016",
doi = "10.1007/7651_2014_127",
language = "English",
isbn = "978-1-4939-2667-1",
volume = "1307",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer Science+Business Media",
pages = "123--140",
booktitle = "Human Embryonic Stem Cell Protocols",
address = "Singapore",

}

RIS

TY - CHAP

T1 - Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways

AU - Vestergaard, Maj Linea

AU - Awan, Aashir

AU - Warzecha, Caroline Becker

AU - Christensen, Søren Tvorup

AU - Andersen, Claus Yding

PY - 2016

Y1 - 2016

N2 - This chapter describes the procedures for immunofluorescence microscopy (IFM) and quantitative PCR (qPCR) analyses of human embryonic stem cells (hESCs) grown specifically under feeder-free conditions. A detailed protocol is provided outlining the steps from initially growing the cells, passaging onto 16-well glass chambers, and continuing with the general IFM and qPCR anlysis. The techniques are illustrated with results on cellular localization of transcriptional factors and components of the Hedgehog, Wnt, PDGF, and TGFβ signaling pathways to primary cilia in stem cell maintenance and differentiation. Furthermore, a sample qPCR experiment is experimentally shown illustrating that these techniques can be important tools in answering basic questions about hESC biology.

AB - This chapter describes the procedures for immunofluorescence microscopy (IFM) and quantitative PCR (qPCR) analyses of human embryonic stem cells (hESCs) grown specifically under feeder-free conditions. A detailed protocol is provided outlining the steps from initially growing the cells, passaging onto 16-well glass chambers, and continuing with the general IFM and qPCR anlysis. The techniques are illustrated with results on cellular localization of transcriptional factors and components of the Hedgehog, Wnt, PDGF, and TGFβ signaling pathways to primary cilia in stem cell maintenance and differentiation. Furthermore, a sample qPCR experiment is experimentally shown illustrating that these techniques can be important tools in answering basic questions about hESC biology.

U2 - 10.1007/7651_2014_127

DO - 10.1007/7651_2014_127

M3 - Book chapter

C2 - 25304206

SN - 978-1-4939-2667-1

SN - 978-1-4939-2668-8

VL - 1307

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 123

EP - 140

BT - Human Embryonic Stem Cell Protocols

PB - Springer Science+Business Media

ER -

ID: 162378679