In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA
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In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA. / Nielsen, M. E.; Rasmussen, I. A.; Kristensen, Stine Gry; Christensen, Søren Tvorup; Møllgård, Kjeld; Andersen, Elisabeth Anne Wreford; Byskov, Anne Grete Sjolte; Andersen, Claus Yding.
I: Molecular Human Reproduction, Bind 17, Nr. 1, 2011, s. 63-70.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA
AU - Nielsen, M. E.
AU - Rasmussen, I. A.
AU - Kristensen, Stine Gry
AU - Christensen, Søren Tvorup
AU - Møllgård, Kjeld
AU - Andersen, Elisabeth Anne Wreford
AU - Byskov, Anne Grete Sjolte
AU - Andersen, Claus Yding
PY - 2011
Y1 - 2011
N2 - Human small antral follicles (diameter of 3-9 mm) were obtained from ovaries surgically removed for fertility preservation. From the individual aspirated follicles, granulosa cells and the corresponding follicular fluid (FF) were isolated in 64 follicles, of which 55 were available for mRNA analysis (24 women). Expression of Androgen Receptor (AR) mRNA levels in granulosa cells, and of androstenedione and testosterone in FF, were correlated to the expression of FSH receptor (FSHR), LH receptor (LHR), CYP19 and anti-Müllerian Hormone-receptor2 (AMHR2) mRNA in the granulosa cells and to the FF concentrations of AMH, inhibin-B, progesterone and estradiol. Androgen Receptor mRNA expression in granulosa cells, and the FF content of androgens, both showed a highly significant positive association with to the expression of FSHR mRNA in granulosa cells. AR mRNA expression also correlated significantly with the expression of AMHR2, but did not correlate with any of the hormones in the FF. These data demonstrate an intimate association between AR expression in immature granulosa cells, and the expression of FSHR in normal small human antral follicles and between the FF levels of androgen and FSHR expression. This suggests that follicular sensitivity towards FSH stimulation may be augmented by stimulation of androgens via the AR.
AB - Human small antral follicles (diameter of 3-9 mm) were obtained from ovaries surgically removed for fertility preservation. From the individual aspirated follicles, granulosa cells and the corresponding follicular fluid (FF) were isolated in 64 follicles, of which 55 were available for mRNA analysis (24 women). Expression of Androgen Receptor (AR) mRNA levels in granulosa cells, and of androstenedione and testosterone in FF, were correlated to the expression of FSH receptor (FSHR), LH receptor (LHR), CYP19 and anti-Müllerian Hormone-receptor2 (AMHR2) mRNA in the granulosa cells and to the FF concentrations of AMH, inhibin-B, progesterone and estradiol. Androgen Receptor mRNA expression in granulosa cells, and the FF content of androgens, both showed a highly significant positive association with to the expression of FSHR mRNA in granulosa cells. AR mRNA expression also correlated significantly with the expression of AMHR2, but did not correlate with any of the hormones in the FF. These data demonstrate an intimate association between AR expression in immature granulosa cells, and the expression of FSHR in normal small human antral follicles and between the FF levels of androgen and FSHR expression. This suggests that follicular sensitivity towards FSH stimulation may be augmented by stimulation of androgens via the AR.
U2 - 10.1093/molehr/gaq073
DO - 10.1093/molehr/gaq073
M3 - Journal article
C2 - 20843821
VL - 17
SP - 63
EP - 70
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
SN - 1360-9947
IS - 1
ER -
ID: 32106692