In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila

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  • Jocelyn L.Y. Tang
  • Krautz, Robert
  • Oriol Llorà-Batlle
  • Anna E. Hakes
  • Paul M. Fox
  • Andrea H. Brand
NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a genetic cross, to the generation of sequencing libraries and, finally, bioinformatic analysis. This protocol can be readily adapted for use in other model systems after simple modifications.
OriginalsprogEngelsk
Artikelnummer101788
TidsskriftSTAR Protocols
Vol/bind3
Udgave nummer4
Antal sider30
ISSN2666-1667
DOI
StatusUdgivet - 2022

Bibliografisk note

Funding Information:
This work was funded by Wellcome Trust PhD Studentships to A.E.H. ( 102454 ) and J.L.Y.T. ( 203798 ), an EMBO Long Term Fellowship to P.M.F. and Wellcome Trust Programme Grant ( 092545 ), Wellcome Trust Senior Investigator Award ( 103792/Z/14/Z ) and Royal Society Darwin Trust Research Professorship ( RP150061 ) to A.H.B. A.H.B. acknowledges core funding to the Gurdon Institute from the Wellcome Trust ( 092096 ) and CRUK ( C6946/A14492 ).

Publisher Copyright:
© 2022 The Authors

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