Isolation of RNP granules

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Standard

Isolation of RNP granules. / Jønson, Lars; Nielsen, Finn Cilius; Christiansen, Jan.

RNA: methods and protocols. red. / Henrik Nielsen. Bind 703 Springer, 2011. s. 265-273 (Methods in Molecular Biology).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Jønson, L, Nielsen, FC & Christiansen, J 2011, Isolation of RNP granules. i H Nielsen (red.), RNA: methods and protocols. bind 703, Springer, Methods in Molecular Biology, s. 265-273. https://doi.org/10.1007/978-1-59745-248-9_18

APA

Jønson, L., Nielsen, F. C., & Christiansen, J. (2011). Isolation of RNP granules. I H. Nielsen (red.), RNA: methods and protocols (Bind 703, s. 265-273). Springer. Methods in Molecular Biology https://doi.org/10.1007/978-1-59745-248-9_18

Vancouver

Jønson L, Nielsen FC, Christiansen J. Isolation of RNP granules. I Nielsen H, red., RNA: methods and protocols. Bind 703. Springer. 2011. s. 265-273. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-248-9_18

Author

Jønson, Lars ; Nielsen, Finn Cilius ; Christiansen, Jan. / Isolation of RNP granules. RNA: methods and protocols. red. / Henrik Nielsen. Bind 703 Springer, 2011. s. 265-273 (Methods in Molecular Biology).

Bibtex

@inbook{da60bd67ece04b6fa9851621a6dbbaf7,
title = "Isolation of RNP granules",
abstract = "The post-transcriptional operon provides a means of synexpression of mRNAs encoding interrelated proteins. The coordination of gene expression may be achieved by a trans-acting RNA-binding protein attaching to similar cis-elements in different, yet functionally clustered, mRNAs. The RNP granule can be regarded as a supramolecular assembly of RNA and protein, probably representing several overlapping post-transcriptional operons. The present protocol describes how RNP granules may be isolated by the transgenic expression of a 3X FLAG version of an RNA-binding protein under tetracycline control via the tetracycline receptor/operator complex. In this way, inclusion of an appropriate tetracycline concentration ensures expression of the tagged version at the endogenous level, and the 3X FLAG tag is a convenient {"}handle{"} for the subsequent immunoprecipitation by immobilized anti-FLAG antibody.",
author = "Lars J{\o}nson and Nielsen, {Finn Cilius} and Jan Christiansen",
year = "2011",
doi = "10.1007/978-1-59745-248-9_18",
language = "English",
isbn = "978-1-58829-913-0",
volume = "703",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "265--273",
editor = "Henrik Nielsen",
booktitle = "RNA",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Isolation of RNP granules

AU - Jønson, Lars

AU - Nielsen, Finn Cilius

AU - Christiansen, Jan

PY - 2011

Y1 - 2011

N2 - The post-transcriptional operon provides a means of synexpression of mRNAs encoding interrelated proteins. The coordination of gene expression may be achieved by a trans-acting RNA-binding protein attaching to similar cis-elements in different, yet functionally clustered, mRNAs. The RNP granule can be regarded as a supramolecular assembly of RNA and protein, probably representing several overlapping post-transcriptional operons. The present protocol describes how RNP granules may be isolated by the transgenic expression of a 3X FLAG version of an RNA-binding protein under tetracycline control via the tetracycline receptor/operator complex. In this way, inclusion of an appropriate tetracycline concentration ensures expression of the tagged version at the endogenous level, and the 3X FLAG tag is a convenient "handle" for the subsequent immunoprecipitation by immobilized anti-FLAG antibody.

AB - The post-transcriptional operon provides a means of synexpression of mRNAs encoding interrelated proteins. The coordination of gene expression may be achieved by a trans-acting RNA-binding protein attaching to similar cis-elements in different, yet functionally clustered, mRNAs. The RNP granule can be regarded as a supramolecular assembly of RNA and protein, probably representing several overlapping post-transcriptional operons. The present protocol describes how RNP granules may be isolated by the transgenic expression of a 3X FLAG version of an RNA-binding protein under tetracycline control via the tetracycline receptor/operator complex. In this way, inclusion of an appropriate tetracycline concentration ensures expression of the tagged version at the endogenous level, and the 3X FLAG tag is a convenient "handle" for the subsequent immunoprecipitation by immobilized anti-FLAG antibody.

U2 - 10.1007/978-1-59745-248-9_18

DO - 10.1007/978-1-59745-248-9_18

M3 - Book chapter

SN - 978-1-58829-913-0

VL - 703

T3 - Methods in Molecular Biology

SP - 265

EP - 273

BT - RNA

A2 - Nielsen, Henrik

PB - Springer

ER -

ID: 40966820