Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms. / Kang, Dingrong; Huang, Yuhong; Nesme, Joseph; Herschend, Jakob; Jacquiod, Samuel; Kot, Witold; Hansen, Lars Hestbjerg; Lange, Lene; Sørensen, Søren J.
I: Science of the Total Environment, Bind 761, 143281, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms
AU - Kang, Dingrong
AU - Huang, Yuhong
AU - Nesme, Joseph
AU - Herschend, Jakob
AU - Jacquiod, Samuel
AU - Kot, Witold
AU - Hansen, Lars Hestbjerg
AU - Lange, Lene
AU - Sørensen, Søren J.
N1 - Copyright © 2020 Elsevier B.V. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Keratin is an insoluble fibrous protein from natural environments, which can be recycled to value-added products by keratinolytic microorganisms. A microbial consortium with efficient keratinolytic activity was previously enriched from soil, but the genetic basis behind its remarkable degradation properties was not investigated yet. To identify the metabolic pathways involved in keratinolysis and clarify the observed synergy among community members, shotgun metagenomic sequencing was performed to reconstruct metagenome-assembled genomes. More than 90% genera of the enriched bacterial consortium was affiliated to Chryseobacterium, Stenotrophomonas, and Pseudomonas. Metabolic potential and putative keratinases were predicted from the metagenomic annotation, providing the genetic basis of keratin degradation. Furthermore, metabolic pathways associated with keratinolytic processes such as amino acid metabolism, disulfide reduction and urea cycle were investigated from seven high-quality metagenome-assembled genomes, revealing the potential metabolic cooperation related to keratin degradation. This knowledge deepens the understanding of microbial keratinolytic mechanisms at play in a complex community, pinpointing the significance of synergistic interactions, which could be further used to optimize industrial keratin degradation processes.
AB - Keratin is an insoluble fibrous protein from natural environments, which can be recycled to value-added products by keratinolytic microorganisms. A microbial consortium with efficient keratinolytic activity was previously enriched from soil, but the genetic basis behind its remarkable degradation properties was not investigated yet. To identify the metabolic pathways involved in keratinolysis and clarify the observed synergy among community members, shotgun metagenomic sequencing was performed to reconstruct metagenome-assembled genomes. More than 90% genera of the enriched bacterial consortium was affiliated to Chryseobacterium, Stenotrophomonas, and Pseudomonas. Metabolic potential and putative keratinases were predicted from the metagenomic annotation, providing the genetic basis of keratin degradation. Furthermore, metabolic pathways associated with keratinolytic processes such as amino acid metabolism, disulfide reduction and urea cycle were investigated from seven high-quality metagenome-assembled genomes, revealing the potential metabolic cooperation related to keratin degradation. This knowledge deepens the understanding of microbial keratinolytic mechanisms at play in a complex community, pinpointing the significance of synergistic interactions, which could be further used to optimize industrial keratin degradation processes.
U2 - 10.1016/j.scitotenv.2020.143281
DO - 10.1016/j.scitotenv.2020.143281
M3 - Journal article
C2 - 33190895
VL - 761
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 143281
ER -
ID: 251737961