Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus

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Standard

Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus. / Deng, Ling; Kenchappa, Chandra Shekar; Peng, Xu; She, Qunxin; Garrett, Roger Antony.

I: Nucleic Acids Research, Bind 40, Nr. 6, 2012, s. 2470-2480.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Deng, L, Kenchappa, CS, Peng, X, She, Q & Garrett, RA 2012, 'Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus', Nucleic Acids Research, bind 40, nr. 6, s. 2470-2480. https://doi.org/10.1093/nar/gkr1111

APA

Deng, L., Kenchappa, C. S., Peng, X., She, Q., & Garrett, R. A. (2012). Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus. Nucleic Acids Research, 40(6), 2470-2480. https://doi.org/10.1093/nar/gkr1111

Vancouver

Deng L, Kenchappa CS, Peng X, She Q, Garrett RA. Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus. Nucleic Acids Research. 2012;40(6):2470-2480. https://doi.org/10.1093/nar/gkr1111

Author

Deng, Ling ; Kenchappa, Chandra Shekar ; Peng, Xu ; She, Qunxin ; Garrett, Roger Antony. / Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus. I: Nucleic Acids Research. 2012 ; Bind 40, Nr. 6. s. 2470-2480.

Bibtex

@article{6ca035b67b82491e843ae9176225e4d8,
title = "Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus",
abstract = "CRISPR loci are essential components of the adaptive immune system of archaea and bacteria. They consist of long arrays of repeats separated by DNA spacers encoding guide RNAs (crRNA), which target foreign genetic elements. Cbp1 (CRISPR DNA repeat binding protein) binds specifically to the multiple direct repeats of CRISPR loci of members of the acidothermophilic, crenarchaeal order Sulfolobales. cbp1 gene deletion from Sulfolobus islandicus REY15A produced a strong reduction in pre-crRNA yields from CRISPR loci but did not inhibit the foreign DNA targeting capacity of the CRISPR/Cas system. Conversely, overexpression of Cbp1 in S. islandicus generated an increase in pre-crRNA yields while the level of reverse strand transcripts from CRISPR loci remained unchanged. It is proposed that Cbp1 modulates production of longer pre-crRNA transcripts from CRISPR loci. A possible mechanism is that it minimizes interference from potential transcriptional signals carried on spacers deriving from A-T-rich genetic elements and, occasionally, on DNA repeats. Supporting evidence is provided by microarray and northern blotting analyses, and publicly available whole-transcriptome data for S. solfataricus P2.",
author = "Ling Deng and Kenchappa, {Chandra Shekar} and Xu Peng and Qunxin She and Garrett, {Roger Antony}",
year = "2012",
doi = "10.1093/nar/gkr1111",
language = "English",
volume = "40",
pages = "2470--2480",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus

AU - Deng, Ling

AU - Kenchappa, Chandra Shekar

AU - Peng, Xu

AU - She, Qunxin

AU - Garrett, Roger Antony

PY - 2012

Y1 - 2012

N2 - CRISPR loci are essential components of the adaptive immune system of archaea and bacteria. They consist of long arrays of repeats separated by DNA spacers encoding guide RNAs (crRNA), which target foreign genetic elements. Cbp1 (CRISPR DNA repeat binding protein) binds specifically to the multiple direct repeats of CRISPR loci of members of the acidothermophilic, crenarchaeal order Sulfolobales. cbp1 gene deletion from Sulfolobus islandicus REY15A produced a strong reduction in pre-crRNA yields from CRISPR loci but did not inhibit the foreign DNA targeting capacity of the CRISPR/Cas system. Conversely, overexpression of Cbp1 in S. islandicus generated an increase in pre-crRNA yields while the level of reverse strand transcripts from CRISPR loci remained unchanged. It is proposed that Cbp1 modulates production of longer pre-crRNA transcripts from CRISPR loci. A possible mechanism is that it minimizes interference from potential transcriptional signals carried on spacers deriving from A-T-rich genetic elements and, occasionally, on DNA repeats. Supporting evidence is provided by microarray and northern blotting analyses, and publicly available whole-transcriptome data for S. solfataricus P2.

AB - CRISPR loci are essential components of the adaptive immune system of archaea and bacteria. They consist of long arrays of repeats separated by DNA spacers encoding guide RNAs (crRNA), which target foreign genetic elements. Cbp1 (CRISPR DNA repeat binding protein) binds specifically to the multiple direct repeats of CRISPR loci of members of the acidothermophilic, crenarchaeal order Sulfolobales. cbp1 gene deletion from Sulfolobus islandicus REY15A produced a strong reduction in pre-crRNA yields from CRISPR loci but did not inhibit the foreign DNA targeting capacity of the CRISPR/Cas system. Conversely, overexpression of Cbp1 in S. islandicus generated an increase in pre-crRNA yields while the level of reverse strand transcripts from CRISPR loci remained unchanged. It is proposed that Cbp1 modulates production of longer pre-crRNA transcripts from CRISPR loci. A possible mechanism is that it minimizes interference from potential transcriptional signals carried on spacers deriving from A-T-rich genetic elements and, occasionally, on DNA repeats. Supporting evidence is provided by microarray and northern blotting analyses, and publicly available whole-transcriptome data for S. solfataricus P2.

U2 - 10.1093/nar/gkr1111

DO - 10.1093/nar/gkr1111

M3 - Journal article

C2 - 22139923

VL - 40

SP - 2470

EP - 2480

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 6

ER -

ID: 37950406