Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water
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Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water. / Kampmeyer, Caroline; Johansen, Jens V.; Holmberg, Christian; Karlson, Magnus; Gersing, Sarah K.; Bordallo, Heloisa N.; Kragelund, Birthe B.; Lerche, Mathilde Hauge; Jourdain, Isabelle; Winther, Jakob R.; Hartmann-Petersen, Rasmus.
I: A C S Synthetic Biology, Bind 9, Nr. 4, 2020, s. 733-748.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water
AU - Kampmeyer, Caroline
AU - Johansen, Jens V.
AU - Holmberg, Christian
AU - Karlson, Magnus
AU - Gersing, Sarah K.
AU - Bordallo, Heloisa N.
AU - Kragelund, Birthe B.
AU - Lerche, Mathilde Hauge
AU - Jourdain, Isabelle
AU - Winther, Jakob R.
AU - Hartmann-Petersen, Rasmus
PY - 2020
Y1 - 2020
N2 - Life is completely dependent on water. To analyze the role of water as a solvent in biology, we replaced water with heavy water (D2O), and investigated the biological effects by a wide range of techniques, using Schizosaccharomyces pombe as model organism. We show that high concentrations of D2O lead to altered glucose metabolism and growth retardation. After prolonged incubation in D2O, cells displayed gross morphological changes, thickened cell walls and aberrant cytoskeletal organization. By transcriptomics and genetic screens, we show that the solvent replacement activates two signaling pathways: 1) the heat-shock response pathway and 2) the cell integrity pathway. Although the heat-shock response system upregulates various chaperones and other stress-relieving enzymes, we find that activation of this pathway does not offer any fitness advantage to the cells under the solvent replaced conditions. However, limiting the D2O-triggered activation of the cell integrity pathway allows cell growth when H2O is completely replaced with D2O. The isolated D2O-tolerant strains may aid biological production of deuterated biomolecules.
AB - Life is completely dependent on water. To analyze the role of water as a solvent in biology, we replaced water with heavy water (D2O), and investigated the biological effects by a wide range of techniques, using Schizosaccharomyces pombe as model organism. We show that high concentrations of D2O lead to altered glucose metabolism and growth retardation. After prolonged incubation in D2O, cells displayed gross morphological changes, thickened cell walls and aberrant cytoskeletal organization. By transcriptomics and genetic screens, we show that the solvent replacement activates two signaling pathways: 1) the heat-shock response pathway and 2) the cell integrity pathway. Although the heat-shock response system upregulates various chaperones and other stress-relieving enzymes, we find that activation of this pathway does not offer any fitness advantage to the cells under the solvent replaced conditions. However, limiting the D2O-triggered activation of the cell integrity pathway allows cell growth when H2O is completely replaced with D2O. The isolated D2O-tolerant strains may aid biological production of deuterated biomolecules.
U2 - 10.1021/acssynbio.9b00376
DO - 10.1021/acssynbio.9b00376
M3 - Journal article
C2 - 32142608
VL - 9
SP - 733
EP - 748
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
SN - 2161-5063
IS - 4
ER -
ID: 237422055