NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system
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NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system. / Tang, Jocelyn L. Y.; Hakes, Anna E.; Krautz, Robert; Suzuki, Takumi; Contreras, Esteban G.; Fox, Paul M.; Brand, Andrea H.
I: Developmental Cell, Bind 57, Nr. 9, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system
AU - Tang, Jocelyn L. Y.
AU - Hakes, Anna E.
AU - Krautz, Robert
AU - Suzuki, Takumi
AU - Contreras, Esteban G.
AU - Fox, Paul M.
AU - Brand, Andrea H.
PY - 2022
Y1 - 2022
N2 - Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot account for the neuronal diversity in the adult brain. To search for missing factors, we developed NanoDam, which enables rapid genome-wide profiling of endogenously tagged proteins in vivo with a single genetic cross. Mapping the targets of known temporal transcription factors with NanoDam revealed that Homeobrain and Scarecrow (ARX and NKX2.1 orthologs) are also temporal factors. We show that Homeobrain and Scarecrow define middle-aged and late INP temporal windows and play a role in cellular longevity. Strikingly, Homeobrain and Scarecrow have conserved functions as temporal factors in the developing visual system. NanoDam enables rapid cell-type-specific genome-wide profiling with temporal resolution and is easily adapted for use in higher organisms.
AB - Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot account for the neuronal diversity in the adult brain. To search for missing factors, we developed NanoDam, which enables rapid genome-wide profiling of endogenously tagged proteins in vivo with a single genetic cross. Mapping the targets of known temporal transcription factors with NanoDam revealed that Homeobrain and Scarecrow (ARX and NKX2.1 orthologs) are also temporal factors. We show that Homeobrain and Scarecrow define middle-aged and late INP temporal windows and play a role in cellular longevity. Strikingly, Homeobrain and Scarecrow have conserved functions as temporal factors in the developing visual system. NanoDam enables rapid cell-type-specific genome-wide profiling with temporal resolution and is easily adapted for use in higher organisms.
KW - OUTER SUBVENTRICULAR ZONE
KW - TARGETED GENE-EXPRESSION
KW - NEURAL STEM-CELLS
KW - NEUROBLAST COMPETENCE
KW - CENTRAL COMPLEX
KW - RADIAL GLIA
KW - LINEAGE
KW - SPECIFICATION
KW - PROGENITORS
KW - GENERATION
U2 - 10.1016/j.devcel.2022.04.008
DO - 10.1016/j.devcel.2022.04.008
M3 - Journal article
C2 - 35483359
VL - 57
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 9
ER -
ID: 333772698