Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli

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Standard

Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli. / Charbon, Godefroid; Bjørn, Louise; Mendoza-Chamizo, Belén; Frimodt-Møller, Jakob; Løbner-Olesen, Anders.

I: Nucleic Acids Research, Bind 42, Nr. 21, 2014, s. 13228-13241.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Charbon, G, Bjørn, L, Mendoza-Chamizo, B, Frimodt-Møller, J & Løbner-Olesen, A 2014, 'Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli', Nucleic Acids Research, bind 42, nr. 21, s. 13228-13241. https://doi.org/10.1093/nar/gku1149

APA

Charbon, G., Bjørn, L., Mendoza-Chamizo, B., Frimodt-Møller, J., & Løbner-Olesen, A. (2014). Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli. Nucleic Acids Research, 42(21), 13228-13241. https://doi.org/10.1093/nar/gku1149

Vancouver

Charbon G, Bjørn L, Mendoza-Chamizo B, Frimodt-Møller J, Løbner-Olesen A. Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli. Nucleic Acids Research. 2014;42(21):13228-13241. https://doi.org/10.1093/nar/gku1149

Author

Charbon, Godefroid ; Bjørn, Louise ; Mendoza-Chamizo, Belén ; Frimodt-Møller, Jakob ; Løbner-Olesen, Anders. / Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli. I: Nucleic Acids Research. 2014 ; Bind 42, Nr. 21. s. 13228-13241.

Bibtex

@article{b9ad3f76be494a3d8bc5df1da9ac0078,
title = "Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli",
abstract = "In Escherichia coli, an increase in the ATP bound form of the DnaA initiator protein results in hyperinitiation and inviability. Here, we show that such replication stress is tolerated during anaerobic growth. In hyperinitiating cells, a shift from anaerobic to aerobic growth resulted in appearance of fragmented chromosomes and a decrease in terminus concentration, leading to a dramatic increase in ori/ter ratio and cessation of cell growth. Aerobic viability was restored by reducing the level of reactive oxygen species (ROS) or by deleting mutM (Fpg glycosylase). The double-strand breaks observed in hyperinitiating cells therefore results from replication forks encountering single-stranded DNA lesions generated while removing oxidized bases, primarily 8-oxoG, from the DNA. We conclude that there is a delicate balance between chromosome replication and ROS inflicted DNA damage so the number of replication forks can only increase when ROS formation is reduced or when the pertinent repair is compromised.",
keywords = "Adenosine Triphosphatases, Aerobiosis, Anaerobiosis, Chromosomes, Bacterial, DNA Breaks, Double-Stranded, DNA Damage, DNA Repair, DNA Replication, DNA, Bacterial, Escherichia coli, Escherichia coli Proteins, Microbial Viability, Mutation, Oxidative Stress",
author = "Godefroid Charbon and Louise Bj{\o}rn and Bel{\'e}n Mendoza-Chamizo and Jakob Frimodt-M{\o}ller and Anders L{\o}bner-Olesen",
note = "{\textcopyright} The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2014",
doi = "10.1093/nar/gku1149",
language = "English",
volume = "42",
pages = "13228--13241",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "21",

}

RIS

TY - JOUR

T1 - Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli

AU - Charbon, Godefroid

AU - Bjørn, Louise

AU - Mendoza-Chamizo, Belén

AU - Frimodt-Møller, Jakob

AU - Løbner-Olesen, Anders

N1 - © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2014

Y1 - 2014

N2 - In Escherichia coli, an increase in the ATP bound form of the DnaA initiator protein results in hyperinitiation and inviability. Here, we show that such replication stress is tolerated during anaerobic growth. In hyperinitiating cells, a shift from anaerobic to aerobic growth resulted in appearance of fragmented chromosomes and a decrease in terminus concentration, leading to a dramatic increase in ori/ter ratio and cessation of cell growth. Aerobic viability was restored by reducing the level of reactive oxygen species (ROS) or by deleting mutM (Fpg glycosylase). The double-strand breaks observed in hyperinitiating cells therefore results from replication forks encountering single-stranded DNA lesions generated while removing oxidized bases, primarily 8-oxoG, from the DNA. We conclude that there is a delicate balance between chromosome replication and ROS inflicted DNA damage so the number of replication forks can only increase when ROS formation is reduced or when the pertinent repair is compromised.

AB - In Escherichia coli, an increase in the ATP bound form of the DnaA initiator protein results in hyperinitiation and inviability. Here, we show that such replication stress is tolerated during anaerobic growth. In hyperinitiating cells, a shift from anaerobic to aerobic growth resulted in appearance of fragmented chromosomes and a decrease in terminus concentration, leading to a dramatic increase in ori/ter ratio and cessation of cell growth. Aerobic viability was restored by reducing the level of reactive oxygen species (ROS) or by deleting mutM (Fpg glycosylase). The double-strand breaks observed in hyperinitiating cells therefore results from replication forks encountering single-stranded DNA lesions generated while removing oxidized bases, primarily 8-oxoG, from the DNA. We conclude that there is a delicate balance between chromosome replication and ROS inflicted DNA damage so the number of replication forks can only increase when ROS formation is reduced or when the pertinent repair is compromised.

KW - Adenosine Triphosphatases

KW - Aerobiosis

KW - Anaerobiosis

KW - Chromosomes, Bacterial

KW - DNA Breaks, Double-Stranded

KW - DNA Damage

KW - DNA Repair

KW - DNA Replication

KW - DNA, Bacterial

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Microbial Viability

KW - Mutation

KW - Oxidative Stress

U2 - 10.1093/nar/gku1149

DO - 10.1093/nar/gku1149

M3 - Journal article

C2 - 25389264

VL - 42

SP - 13228

EP - 13241

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 21

ER -

ID: 131364647