Physiological studies on the effect of Ca2+ on the duration of the lag phase of Saccharomyces cerevisiae
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Physiological studies on the effect of Ca2+ on the duration of the lag phase of Saccharomyces cerevisiae. / Friis, J; Christensen, Søren Tvorup; Szablewski, L; Schousboe, P; Rasmussen, L.
I: FEMS Microbiology Letters, Bind 123, Nr. 1-2, 1994, s. 33-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Physiological studies on the effect of Ca2+ on the duration of the lag phase of Saccharomyces cerevisiae
AU - Friis, J
AU - Christensen, Søren Tvorup
AU - Szablewski, L
AU - Schousboe, P
AU - Rasmussen, L
N1 - Keywords: Calcimycin; Calcium; Culture Media; Saccharomyces cerevisiae
PY - 1994
Y1 - 1994
N2 - Cell multiplication and growth of Saccharomyces cerevisiae were followed in 2-ml test tubes containing Wickerham's synthetic medium or very dilute synthetic media supplemented in various ways. The ability of the cell cultures to leave the lag phase and enter the exponential phase of growth was investigated. Multiplication was assessed by microscopical observation. The results showed great differences in times required for the cultures to leave the lag phases and begin multiplication. In Wickerham's medium, all cultures grew well 6 h after inoculation. In the dilute medium, several days elapsed before all the cultures grew. These cultures went into exponential growth with approximately first order kinetics. In the unsupplemented medium, the 'half-lives' in the lag phase were about 28 h. Addition of either Ca2+ or Ca2+ plus A23187 (calcimycin) reduced the half-lives to 10 and 6 h, respectively. The doubling times in the exponential phases of growth were not shortened by these additions. We suggest that Ca2+ plays a crucial role as a signal to switch on the mode of cell proliferation in S. cerevisiae.
AB - Cell multiplication and growth of Saccharomyces cerevisiae were followed in 2-ml test tubes containing Wickerham's synthetic medium or very dilute synthetic media supplemented in various ways. The ability of the cell cultures to leave the lag phase and enter the exponential phase of growth was investigated. Multiplication was assessed by microscopical observation. The results showed great differences in times required for the cultures to leave the lag phases and begin multiplication. In Wickerham's medium, all cultures grew well 6 h after inoculation. In the dilute medium, several days elapsed before all the cultures grew. These cultures went into exponential growth with approximately first order kinetics. In the unsupplemented medium, the 'half-lives' in the lag phase were about 28 h. Addition of either Ca2+ or Ca2+ plus A23187 (calcimycin) reduced the half-lives to 10 and 6 h, respectively. The doubling times in the exponential phases of growth were not shortened by these additions. We suggest that Ca2+ plays a crucial role as a signal to switch on the mode of cell proliferation in S. cerevisiae.
U2 - 10.1111/j.1574-6968.1994.tb07197.x
DO - 10.1111/j.1574-6968.1994.tb07197.x
M3 - Journal article
C2 - 7988895
VL - 123
SP - 33
EP - 36
JO - F E M S Microbiology Letters
JF - F E M S Microbiology Letters
SN - 0378-1097
IS - 1-2
ER -
ID: 11255991