Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters

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Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters. / Chen, Yun; Pai, Athma A; Herudek, Jan; Lubas, Michal; Meola, Nicola; Järvelin, Aino I.; Andersson, Robin; Pelechano, Vicent; Steinmetz, Lars M.; Jensen, Torben Heick; Sandelin, Albin Gustav.

I: Nature Genetics, Bind 48, Nr. 9, 2016, s. 984-994.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Chen, Y, Pai, AA, Herudek, J, Lubas, M, Meola, N, Järvelin, AI, Andersson, R, Pelechano, V, Steinmetz, LM, Jensen, TH & Sandelin, AG 2016, 'Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters', Nature Genetics, bind 48, nr. 9, s. 984-994. https://doi.org/10.1038/ng.3616

APA

Chen, Y., Pai, A. A., Herudek, J., Lubas, M., Meola, N., Järvelin, A. I., Andersson, R., Pelechano, V., Steinmetz, L. M., Jensen, T. H., & Sandelin, A. G. (2016). Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters. Nature Genetics, 48(9), 984-994. https://doi.org/10.1038/ng.3616

Vancouver

Chen Y, Pai AA, Herudek J, Lubas M, Meola N, Järvelin AI o.a. Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters. Nature Genetics. 2016;48(9):984-994. https://doi.org/10.1038/ng.3616

Author

Chen, Yun ; Pai, Athma A ; Herudek, Jan ; Lubas, Michal ; Meola, Nicola ; Järvelin, Aino I. ; Andersson, Robin ; Pelechano, Vicent ; Steinmetz, Lars M. ; Jensen, Torben Heick ; Sandelin, Albin Gustav. / Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters. I: Nature Genetics. 2016 ; Bind 48, Nr. 9. s. 984-994.

Bibtex

@article{1bcb627a60fe40bb8093a9540c807bff,
title = "Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters",
abstract = "Mammalian transcriptomes are complex and formed by extensive promoter activity. In addition, gene promoters are largely divergent and initiate transcription of reverse-oriented promoter upstream transcripts (PROMPTs). Although PROMPTs are commonly terminated early, influenced by polyadenylation sites, promoters often cluster so that the divergent activity of one might impact another. Here we found that the distance between promoters strongly correlates with the expression, stability and length of their associated PROMPTs. Adjacent promoters driving divergent mRNA transcription support PROMPT formation, but owing to polyadenylation site constraints, these transcripts tend to spread into the neighboring mRNA on the same strand. This mechanism to derive new alternative mRNA transcription start sites (TSSs) is also evident at closely spaced promoters supporting convergent mRNA transcription. We suggest that basic building blocks of divergently transcribed core promoter pairs, in combination with the wealth of TSSs in mammalian genomes, provide a framework with which evolution shapes transcriptomes.",
keywords = "Journal Article",
author = "Yun Chen and Pai, {Athma A} and Jan Herudek and Michal Lubas and Nicola Meola and J{\"a}rvelin, {Aino I.} and Robin Andersson and Vicent Pelechano and Steinmetz, {Lars M.} and Jensen, {Torben Heick} and Sandelin, {Albin Gustav}",
year = "2016",
doi = "10.1038/ng.3616",
language = "English",
volume = "48",
pages = "984--994",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "nature publishing group",
number = "9",

}

RIS

TY - JOUR

T1 - Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters

AU - Chen, Yun

AU - Pai, Athma A

AU - Herudek, Jan

AU - Lubas, Michal

AU - Meola, Nicola

AU - Järvelin, Aino I.

AU - Andersson, Robin

AU - Pelechano, Vicent

AU - Steinmetz, Lars M.

AU - Jensen, Torben Heick

AU - Sandelin, Albin Gustav

PY - 2016

Y1 - 2016

N2 - Mammalian transcriptomes are complex and formed by extensive promoter activity. In addition, gene promoters are largely divergent and initiate transcription of reverse-oriented promoter upstream transcripts (PROMPTs). Although PROMPTs are commonly terminated early, influenced by polyadenylation sites, promoters often cluster so that the divergent activity of one might impact another. Here we found that the distance between promoters strongly correlates with the expression, stability and length of their associated PROMPTs. Adjacent promoters driving divergent mRNA transcription support PROMPT formation, but owing to polyadenylation site constraints, these transcripts tend to spread into the neighboring mRNA on the same strand. This mechanism to derive new alternative mRNA transcription start sites (TSSs) is also evident at closely spaced promoters supporting convergent mRNA transcription. We suggest that basic building blocks of divergently transcribed core promoter pairs, in combination with the wealth of TSSs in mammalian genomes, provide a framework with which evolution shapes transcriptomes.

AB - Mammalian transcriptomes are complex and formed by extensive promoter activity. In addition, gene promoters are largely divergent and initiate transcription of reverse-oriented promoter upstream transcripts (PROMPTs). Although PROMPTs are commonly terminated early, influenced by polyadenylation sites, promoters often cluster so that the divergent activity of one might impact another. Here we found that the distance between promoters strongly correlates with the expression, stability and length of their associated PROMPTs. Adjacent promoters driving divergent mRNA transcription support PROMPT formation, but owing to polyadenylation site constraints, these transcripts tend to spread into the neighboring mRNA on the same strand. This mechanism to derive new alternative mRNA transcription start sites (TSSs) is also evident at closely spaced promoters supporting convergent mRNA transcription. We suggest that basic building blocks of divergently transcribed core promoter pairs, in combination with the wealth of TSSs in mammalian genomes, provide a framework with which evolution shapes transcriptomes.

KW - Journal Article

U2 - 10.1038/ng.3616

DO - 10.1038/ng.3616

M3 - Journal article

C2 - 27455346

VL - 48

SP - 984

EP - 994

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 9

ER -

ID: 165390237