Production of a heterologous proteinase A by Saccharomyces kluyveri

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Standard

Production of a heterologous proteinase A by Saccharomyces kluyveri. / Møller, K.; Tidemand, L. D.; Winther, J. R.; Olsson, L.; Piskur, J.; Nielsen, J.

I: Applied Microbiology and Biotechnology, Bind 57, Nr. 1-2, 2001, s. 216-219.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Møller, K, Tidemand, LD, Winther, JR, Olsson, L, Piskur, J & Nielsen, J 2001, 'Production of a heterologous proteinase A by Saccharomyces kluyveri', Applied Microbiology and Biotechnology, bind 57, nr. 1-2, s. 216-219. https://doi.org/10.1007/s002530100680

APA

Møller, K., Tidemand, L. D., Winther, J. R., Olsson, L., Piskur, J., & Nielsen, J. (2001). Production of a heterologous proteinase A by Saccharomyces kluyveri. Applied Microbiology and Biotechnology, 57(1-2), 216-219. https://doi.org/10.1007/s002530100680

Vancouver

Møller K, Tidemand LD, Winther JR, Olsson L, Piskur J, Nielsen J. Production of a heterologous proteinase A by Saccharomyces kluyveri. Applied Microbiology and Biotechnology. 2001;57(1-2):216-219. https://doi.org/10.1007/s002530100680

Author

Møller, K. ; Tidemand, L. D. ; Winther, J. R. ; Olsson, L. ; Piskur, J. ; Nielsen, J. / Production of a heterologous proteinase A by Saccharomyces kluyveri. I: Applied Microbiology and Biotechnology. 2001 ; Bind 57, Nr. 1-2. s. 216-219.

Bibtex

@article{f386bc2379f44635b883a7d5bba69ba1,
title = "Production of a heterologous proteinase A by Saccharomyces kluyveri",
abstract = "In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.",
keywords = "Aspartic Acid Endopeptidases, Ethanol, Glucose, Plasmids, Promoter Regions, Genetic, Saccharomyces",
author = "K. M{\o}ller and Tidemand, {L. D.} and Winther, {J. R.} and L. Olsson and J. Piskur and J. Nielsen",
year = "2001",
doi = "10.1007/s002530100680",
language = "English",
volume = "57",
pages = "216--219",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer",
number = "1-2",

}

RIS

TY - JOUR

T1 - Production of a heterologous proteinase A by Saccharomyces kluyveri

AU - Møller, K.

AU - Tidemand, L. D.

AU - Winther, J. R.

AU - Olsson, L.

AU - Piskur, J.

AU - Nielsen, J.

PY - 2001

Y1 - 2001

N2 - In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.

AB - In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.

KW - Aspartic Acid Endopeptidases

KW - Ethanol

KW - Glucose

KW - Plasmids

KW - Promoter Regions, Genetic

KW - Saccharomyces

U2 - 10.1007/s002530100680

DO - 10.1007/s002530100680

M3 - Journal article

C2 - 11693924

VL - 57

SP - 216

EP - 219

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 1-2

ER -

ID: 43973662