RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40. / Simpson, Laura J.; Ross, Anna Laura; Szüts, Dávid; Alviani, Cherry A.; Oestergaard, Vibe H.; Patel, Ketan J.; Sale, Julian E.

I: EMBO Reports, Bind 7, Nr. 9, 01.09.2006, s. 927-932.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Simpson, LJ, Ross, AL, Szüts, D, Alviani, CA, Oestergaard, VH, Patel, KJ & Sale, JE 2006, 'RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40', EMBO Reports, bind 7, nr. 9, s. 927-932. https://doi.org/10.1038/sj.embor.7400777

APA

Simpson, L. J., Ross, A. L., Szüts, D., Alviani, C. A., Oestergaard, V. H., Patel, K. J., & Sale, J. E. (2006). RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40. EMBO Reports, 7(9), 927-932. https://doi.org/10.1038/sj.embor.7400777

Vancouver

Simpson LJ, Ross AL, Szüts D, Alviani CA, Oestergaard VH, Patel KJ o.a. RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40. EMBO Reports. 2006 sep. 1;7(9):927-932. https://doi.org/10.1038/sj.embor.7400777

Author

Simpson, Laura J. ; Ross, Anna Laura ; Szüts, Dávid ; Alviani, Cherry A. ; Oestergaard, Vibe H. ; Patel, Ketan J. ; Sale, Julian E. / RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40. I: EMBO Reports. 2006 ; Bind 7, Nr. 9. s. 927-932.

Bibtex

@article{130dd0589720421daa1ebf209ea4272e,
title = "RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40",
abstract = "Ubiquitination of proliferating-cell nuclear antigen (PCNA) at K164 by RAD6/RAD18 has a key role in DNA damage tolerance in yeast. Here, we report on the first genetic study of this modification in a vertebrate cell. As in yeast, mutation of K164 of PCNA to arginine in the avian cell line DT40 results in sensitivity to DNA damage but, by contrast, the DT40 pcnaK164R mutant is more sensitive than the rad18 mutant. Consistent with this, we show the presence of residual ubiquitination of PCNA at K164 in the absence of functional RAD18, suggesting the presence of an alternate PCNA ubiquitinating enzyme in DT40. Furthermore, RAD18 and PCNA K164 have non-overlapping roles in the suppression of sister chromatid exchange in DT40, showing that RAD18 has other functions that do not involve the ubiquitination of PCNA.",
author = "Simpson, {Laura J.} and Ross, {Anna Laura} and D{\'a}vid Sz{\"u}ts and Alviani, {Cherry A.} and Oestergaard, {Vibe H.} and Patel, {Ketan J.} and Sale, {Julian E.}",
year = "2006",
month = sep,
day = "1",
doi = "10.1038/sj.embor.7400777",
language = "English",
volume = "7",
pages = "927--932",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell",
number = "9",

}

RIS

TY - JOUR

T1 - RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40

AU - Simpson, Laura J.

AU - Ross, Anna Laura

AU - Szüts, Dávid

AU - Alviani, Cherry A.

AU - Oestergaard, Vibe H.

AU - Patel, Ketan J.

AU - Sale, Julian E.

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Ubiquitination of proliferating-cell nuclear antigen (PCNA) at K164 by RAD6/RAD18 has a key role in DNA damage tolerance in yeast. Here, we report on the first genetic study of this modification in a vertebrate cell. As in yeast, mutation of K164 of PCNA to arginine in the avian cell line DT40 results in sensitivity to DNA damage but, by contrast, the DT40 pcnaK164R mutant is more sensitive than the rad18 mutant. Consistent with this, we show the presence of residual ubiquitination of PCNA at K164 in the absence of functional RAD18, suggesting the presence of an alternate PCNA ubiquitinating enzyme in DT40. Furthermore, RAD18 and PCNA K164 have non-overlapping roles in the suppression of sister chromatid exchange in DT40, showing that RAD18 has other functions that do not involve the ubiquitination of PCNA.

AB - Ubiquitination of proliferating-cell nuclear antigen (PCNA) at K164 by RAD6/RAD18 has a key role in DNA damage tolerance in yeast. Here, we report on the first genetic study of this modification in a vertebrate cell. As in yeast, mutation of K164 of PCNA to arginine in the avian cell line DT40 results in sensitivity to DNA damage but, by contrast, the DT40 pcnaK164R mutant is more sensitive than the rad18 mutant. Consistent with this, we show the presence of residual ubiquitination of PCNA at K164 in the absence of functional RAD18, suggesting the presence of an alternate PCNA ubiquitinating enzyme in DT40. Furthermore, RAD18 and PCNA K164 have non-overlapping roles in the suppression of sister chromatid exchange in DT40, showing that RAD18 has other functions that do not involve the ubiquitination of PCNA.

UR - http://www.scopus.com/inward/record.url?scp=33749510488&partnerID=8YFLogxK

U2 - 10.1038/sj.embor.7400777

DO - 10.1038/sj.embor.7400777

M3 - Journal article

C2 - 16888649

AN - SCOPUS:33749510488

VL - 7

SP - 927

EP - 932

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

IS - 9

ER -

ID: 238744187