Regulation of taurine homeostasis by protein kinase CK2 in mouse fibroblasts
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Regulation of taurine homeostasis by protein kinase CK2 in mouse fibroblasts. / Hansen, Daniel Bloch; Guerra, Barbara; Jacobsen, Jack Hummeland ; Lambert, Ian Henry.
I: Amino Acids, Bind 40, Nr. 4, 2011, s. 1091-1106.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Regulation of taurine homeostasis by protein kinase CK2 in mouse fibroblasts
AU - Hansen, Daniel Bloch
AU - Guerra, Barbara
AU - Jacobsen, Jack Hummeland
AU - Lambert, Ian Henry
PY - 2011
Y1 - 2011
N2 - Increased expression of the ubiquitous serine/threonine protein kinase CK2 has been associated with increased proliferative capacity and increased resistance towards apoptosis. Taurine is the primary organic osmolyte involved in cell volume control in mammalian cells, and shift in cell volume is a critical step in cell proliferation, differentiation and induction of apoptosis. In the present study, we use mouse NIH3T3 fibroblasts and Ehrlich Lettré ascites tumour cells with different CK2 expression levels. Taurine uptake via the Na(+) dependent transporter TauT and taurine release are increased and reduced, respectively, following pharmacological CK2 inhibition. The effect of CK2 inhibition on TauT involves modulation of transport kinetics, whereas the effect on the taurine release pathway involves reduction in the open-probability of the efflux pathway. Stimulation of PLA(2) activity, exposure to exogenous reactive oxygen species as well as inhibition of protein tyrosine phosphotases (PTP) potentiate the swelling-induced taurine loss. Inhibition of PI3K and PTEN reduces and potentiates swelling-induced taurine release, respectively. Inhibition of CK2 has no effect on PLA(2) activity and ROS production by NADPH oxidase, whereas it lifts the effect of PTEN and PTP inhibition. It is suggested that CK2 regulates the taurine release downstream to known swelling-induced signal transducers including PLA(2), NADPH oxidase and PI3K.
AB - Increased expression of the ubiquitous serine/threonine protein kinase CK2 has been associated with increased proliferative capacity and increased resistance towards apoptosis. Taurine is the primary organic osmolyte involved in cell volume control in mammalian cells, and shift in cell volume is a critical step in cell proliferation, differentiation and induction of apoptosis. In the present study, we use mouse NIH3T3 fibroblasts and Ehrlich Lettré ascites tumour cells with different CK2 expression levels. Taurine uptake via the Na(+) dependent transporter TauT and taurine release are increased and reduced, respectively, following pharmacological CK2 inhibition. The effect of CK2 inhibition on TauT involves modulation of transport kinetics, whereas the effect on the taurine release pathway involves reduction in the open-probability of the efflux pathway. Stimulation of PLA(2) activity, exposure to exogenous reactive oxygen species as well as inhibition of protein tyrosine phosphotases (PTP) potentiate the swelling-induced taurine loss. Inhibition of PI3K and PTEN reduces and potentiates swelling-induced taurine release, respectively. Inhibition of CK2 has no effect on PLA(2) activity and ROS production by NADPH oxidase, whereas it lifts the effect of PTEN and PTP inhibition. It is suggested that CK2 regulates the taurine release downstream to known swelling-induced signal transducers including PLA(2), NADPH oxidase and PI3K.
KW - Animals
KW - Apoptosis
KW - Biological Transport
KW - Carcinoma, Ehrlich Tumor
KW - Casein Kinase II
KW - Cell Size
KW - Fibroblasts
KW - Gene Expression
KW - Homeostasis
KW - Kinetics
KW - Mice
KW - NADPH Oxidase
KW - NIH 3T3 Cells
KW - PTEN Phosphohydrolase
KW - Phosphatidylinositol 3-Kinases
KW - Phospholipases A2
KW - Protein Kinase Inhibitors
KW - Protein Tyrosine Phosphatases
KW - Reactive Oxygen Species
KW - Signal Transduction
KW - Taurine
KW - Tumor Cells, Cultured
KW - Water-Electrolyte Balance
U2 - 10.1007/s00726-010-0732-y
DO - 10.1007/s00726-010-0732-y
M3 - Journal article
C2 - 20827495
VL - 40
SP - 1091
EP - 1106
JO - Amino Acids
JF - Amino Acids
SN - 0939-4451
IS - 4
ER -
ID: 33884700