Seawater-regulated genes for two-component systems and outer membrane proteins in myxococcus
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Seawater-regulated genes for two-component systems and outer membrane proteins in myxococcus. / Pan, Hong Wei; Liu, Hong; Liu, Ting; Li, Cheng Yun; Li, Zhi Feng; Cai, Ke; Zhang, Cui Ying; Zhang, Yong; Hu, Wei; Wu, Zhi Hong; Li, Yue Zhong.
I: Journal of Bacteriology, Bind 191, Nr. 7, 04.2009, s. 2102-2111.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Seawater-regulated genes for two-component systems and outer membrane proteins in myxococcus
AU - Pan, Hong Wei
AU - Liu, Hong
AU - Liu, Ting
AU - Li, Cheng Yun
AU - Li, Zhi Feng
AU - Cai, Ke
AU - Zhang, Cui Ying
AU - Zhang, Yong
AU - Hu, Wei
AU - Wu, Zhi Hong
AU - Li, Yue Zhong
PY - 2009/4
Y1 - 2009/4
N2 - When salt-tolerant Myxococcus cells are moved to a seawater environment, they change their growth, morphology, and developmental behavior. Outer membrane proteins and signal transduction pathways may play important roles in this shift. Chip hybridization targeting the genes predicted to encode 226 twocomponent signal transduction pathways and 74 outer membrane proteins of M. xanthus DK1622 revealed that the expression of 55 corresponding genes in the salt-tolerant strain M. fulvus HW-1 was significantly modified (most were downregulated) by the presence of seawater. Sequencing revealed that these seawater-regulated genes are highly homologous in both strains, suggesting that they have similar roles in the lifestyle of Myxococcus. Seven of the genes that had been reported in M. xanthus DK1622 are involved in different cellular processes, such as fruiting body development, sporulation, or motility. The outer membrane (Om) gene Om031 had the most significant change in expression (downregulated) in response to seawater, while the twocomponent system (Tc) gene Tc105 had the greatest increase in expression. Their homologues MXAN3106 and MXAN4042 were knocked out in DK1622 to analyze their functions in response to changes in salinity. In addition to having increased salt tolerance, sporulation of the MXAN3106 mutant was enhanced compared to that of DK1622, whereas mutating gene MXAN4042 produced contrary results. The results indicated that the genes that are involved in the cellular processes that are significantly changed in response to salinity may also be involved the salt tolerance of Myxococcus cells. Regulating the expression levels of these multifunctional genes may allow cells to quickly and efficiently respond to changing conditions in coastal environments.
AB - When salt-tolerant Myxococcus cells are moved to a seawater environment, they change their growth, morphology, and developmental behavior. Outer membrane proteins and signal transduction pathways may play important roles in this shift. Chip hybridization targeting the genes predicted to encode 226 twocomponent signal transduction pathways and 74 outer membrane proteins of M. xanthus DK1622 revealed that the expression of 55 corresponding genes in the salt-tolerant strain M. fulvus HW-1 was significantly modified (most were downregulated) by the presence of seawater. Sequencing revealed that these seawater-regulated genes are highly homologous in both strains, suggesting that they have similar roles in the lifestyle of Myxococcus. Seven of the genes that had been reported in M. xanthus DK1622 are involved in different cellular processes, such as fruiting body development, sporulation, or motility. The outer membrane (Om) gene Om031 had the most significant change in expression (downregulated) in response to seawater, while the twocomponent system (Tc) gene Tc105 had the greatest increase in expression. Their homologues MXAN3106 and MXAN4042 were knocked out in DK1622 to analyze their functions in response to changes in salinity. In addition to having increased salt tolerance, sporulation of the MXAN3106 mutant was enhanced compared to that of DK1622, whereas mutating gene MXAN4042 produced contrary results. The results indicated that the genes that are involved in the cellular processes that are significantly changed in response to salinity may also be involved the salt tolerance of Myxococcus cells. Regulating the expression levels of these multifunctional genes may allow cells to quickly and efficiently respond to changing conditions in coastal environments.
UR - http://www.scopus.com/inward/record.url?scp=64049112239&partnerID=8YFLogxK
U2 - 10.1128/JB.01556-08
DO - 10.1128/JB.01556-08
M3 - Journal article
C2 - 19151139
AN - SCOPUS:64049112239
VL - 191
SP - 2102
EP - 2111
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 7
ER -
ID: 298631406