Soil texture is a stronger driver of the maize rhizosphere microbiome and extracellular enzyme activities than soil depth or the presence of root hairs
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Different drivers are known to shape rhizosphere microbiome assembly. How soil texture (Texture) and presence or lack of root hairs (Root Hair) of plants affect the rhizosphere microbiome assembly and soil potential extracellular enzyme activities (EEA) at defined rooting depth (Depth) is still a knowledge gap. We investigated effects of these drivers on microbial assembly in rhizosphere and on potential EEA in root-affected soil of maize.
Methods
Samples were taken from three depths of root hair defective mutant rth3 and wild-type WT maize planted on loam and sand in soil columns after 22 days. Rhizosphere bacterial, archaeal, fungal and cercozoan communities were analysed by sequencing of 16S rRNA gene, ITS and 18S rRNA gene fragments. Soil potential EEA of ß-glucosidase, acid phosphatase and chitinase were estimated using fluorogenic substrates.
Results
The bacterial, archaeal and cercozoan alpha- and beta-diversities were significantly and strongly altered by Texture, followed by Depth and Root Hair. Texture and Depth had a small impact on fungal assembly, and only fungal beta-diversity was significantly affected. Significant impacts by Depth and Root Hair on beta-diversity and relative abundances at taxonomic levels of bacteria, archaea, fungi and cercozoa were dependent on Texture. Likewise, the patterns of potential EEA followed the trends of microbial communities, and the potential EEA correlated with the relative abundances of several taxa.
Conclusions
Texture was the strongest driver of rhizosphere microbiome and of soil potential EEA, followed by Depth and Root Hair, similarly to findings in maize root architecture and plant gene expression studies.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Plant and Soil |
| Vol/bind | 478 |
| Udgave nummer | 1-2 |
| Sider (fra-til) | 229-251 |
| Antal sider | 23 |
| ISSN | 0032-079X |
| DOI | |
| Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
This project was carried out in the framework of the priority program 2089 “Rhizosphere spatiotemporal organization - a key to rhizosphere functions” funded by DFG (Deutsche Forschungsgemeinschaft), project numbers 403637238, 403640293, 403641192, 403664478 and 403635931. Seeds of the maize WT and rth3 were provided by Caroline Marcon and Frank Hochholdinger (University of Bonn). We also would like to acknowledge all participants involved in the sampling at UFZ Halle, Dr. Doreen Babin and Ilse-Marie Jungkurth for reading the MS.
Funding Information:
This project was carried out in the framework of the priority program 2089 “Rhizosphere spatiotemporal organization - a key to rhizosphere functions” funded by DFG (Deutsche Forschungsgemeinschaft), project numbers 403637238, 403640293, 403641192, 403664478 and 403635931. Seeds of the maize WT and rth3 were provided by Caroline Marcon and Frank Hochholdinger (University of Bonn). We also would like to acknowledge all participants involved in the sampling at UFZ Halle, Dr. Doreen Babin and Ilse-Marie Jungkurth for reading the MS.
Funding Information:
Open Access funding enabled and organized by Projekt DEAL. This research was conducted within the research program “Rhizosphere Spatiotemporal Organization – a Key to Rhizosphere Functions” of the German Science Foundation, funded by the Deutsche Forschungsgemeinschaft (project numbers 403637238, 403640293, 403641192, 403664478 and 403635931). AHB was supported by the German Centre for Integrative Biodiversity Research (iDiv) Halle–Jena–Leipzig, funded by the Deutsche Forschungsgemeinschaft (DFG-FZT 118, 202548816).
Publisher Copyright:
© 2022, The Author(s).
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