Systematic Analysis of Long Non-Coding RNAs in Inflammasome Activation in Monocytes/Macrophages
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Systematic Analysis of Long Non-Coding RNAs in Inflammasome Activation in Monocytes/Macrophages. / Qian, Na; Distefano, Rebecca; Ilieva, Mirolyuba; Madsen, Jens Hedelund; Rennie, Sarah; Uchida, Shizuka.
I: Non-coding RNA, Bind 9, Nr. 5, 50, 2023.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Systematic Analysis of Long Non-Coding RNAs in Inflammasome Activation in Monocytes/Macrophages
AU - Qian, Na
AU - Distefano, Rebecca
AU - Ilieva, Mirolyuba
AU - Madsen, Jens Hedelund
AU - Rennie, Sarah
AU - Uchida, Shizuka
N1 - Publisher Copyright: © 2023 by the authors.
PY - 2023
Y1 - 2023
N2 - The NLRP3 inflammasome plays a pivotal role in regulating inflammation and immune responses. Its activation can lead to an inflammatory response and pyroptotic cell death. This is beneficial in the case of infections, but excessive activation can lead to chronic inflammation and tissue damage. Moreover, while most of the mammalian genome is transcribed as RNAs, only a small fraction codes for proteins. Among non-protein-coding RNAs, long non-coding RNAs (lncRNAs) have been shown to play key roles in regulating gene expression and cellular processes. They interact with DNA, RNAs, and proteins, and their dysregulation can provide insights into disease mechanisms, including NLRP3 inflammasome activation. Here, we systematically analyzed previously published RNA sequencing (RNA-seq) data of NLRP3 inflammasome activation in monocytes/macrophages to uncover inflammasome-regulated lncRNA genes. To uncover the functional importance of inflammasome-regulated lncRNA genes, one inflammasome-regulated lncRNA, ENSG00000273124, was knocked down in an in vitro model of macrophage polarization. The results indicate that silencing of ENSG00000273124 resulted in the up-regulation tumor necrosis factor (TNF), suggesting that this lncRNA might be involved in pro-inflammatory response in macrophages. To make our analyzed data more accessible, we developed the web database InflammasomeDB.
AB - The NLRP3 inflammasome plays a pivotal role in regulating inflammation and immune responses. Its activation can lead to an inflammatory response and pyroptotic cell death. This is beneficial in the case of infections, but excessive activation can lead to chronic inflammation and tissue damage. Moreover, while most of the mammalian genome is transcribed as RNAs, only a small fraction codes for proteins. Among non-protein-coding RNAs, long non-coding RNAs (lncRNAs) have been shown to play key roles in regulating gene expression and cellular processes. They interact with DNA, RNAs, and proteins, and their dysregulation can provide insights into disease mechanisms, including NLRP3 inflammasome activation. Here, we systematically analyzed previously published RNA sequencing (RNA-seq) data of NLRP3 inflammasome activation in monocytes/macrophages to uncover inflammasome-regulated lncRNA genes. To uncover the functional importance of inflammasome-regulated lncRNA genes, one inflammasome-regulated lncRNA, ENSG00000273124, was knocked down in an in vitro model of macrophage polarization. The results indicate that silencing of ENSG00000273124 resulted in the up-regulation tumor necrosis factor (TNF), suggesting that this lncRNA might be involved in pro-inflammatory response in macrophages. To make our analyzed data more accessible, we developed the web database InflammasomeDB.
KW - inflammasome
KW - lncRNA
KW - macrophages
KW - RNA-seq
U2 - 10.3390/ncrna9050050
DO - 10.3390/ncrna9050050
M3 - Journal article
C2 - 37736896
AN - SCOPUS:85175321569
VL - 9
JO - Non-coding RNA
JF - Non-coding RNA
SN - 2311-553X
IS - 5
M1 - 50
ER -
ID: 372182772