The B-domain of factor VIII reduces cell membrane attachement to host cells in serum free conditions
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
The B-domain of factor VIII reduces cell membrane attachement to host cells in serum free conditions. / Kolind, Mille Petersen; Nørby, Peder Lisby; Flintegaard, Thomas Veje; Berchtold, Martin Werner; Johnsen, Laust Bruun.
I: Journal of Biotechnology, Bind 147, Nr. 3-4, 2010, s. 198-204.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The B-domain of factor VIII reduces cell membrane attachement to host cells in serum free conditions
AU - Kolind, Mille Petersen
AU - Nørby, Peder Lisby
AU - Flintegaard, Thomas Veje
AU - Berchtold, Martin Werner
AU - Johnsen, Laust Bruun
PY - 2010
Y1 - 2010
N2 - Factor VIII (FVIII) is an important protein in the blood coagulation cascade and dysfunction or deficiency of FVIII causes haemophilia A. Replacement therapy with exogenous recombinant FVIII (rFVIII) works as a substitute for the missing or non-functioning FVIII. The rFVIII protein has been engineered extensively throughout the years to increase the low production yields that initially were obtained from mammalian cell cultures.The scope of this work was to investigate the interaction of rFVIII with the cell membrane surface of the producing cells in serum free medium. We wondered whether binding of rFVIII to the cell membrane could be a factor diminishing the production yield. We studied the contribution of the rFVIII B-domain to membrane attachment by transfecting several constructs containing increasing lengths of the B-domain into cells under serum free conditions. We found that 90% of rFVIII is attached to the cell membrane of the producing cell when the rFVIII variant contains a short B-domain (21 aa). By increasing the length of the B-domain the membrane attached fraction can be reduced to 50% of the total expressed rFVIII. Further, our studies show that the N-linked glycosylations within the B-domain have no influence on either total expression level or membrane attachment properties
AB - Factor VIII (FVIII) is an important protein in the blood coagulation cascade and dysfunction or deficiency of FVIII causes haemophilia A. Replacement therapy with exogenous recombinant FVIII (rFVIII) works as a substitute for the missing or non-functioning FVIII. The rFVIII protein has been engineered extensively throughout the years to increase the low production yields that initially were obtained from mammalian cell cultures.The scope of this work was to investigate the interaction of rFVIII with the cell membrane surface of the producing cells in serum free medium. We wondered whether binding of rFVIII to the cell membrane could be a factor diminishing the production yield. We studied the contribution of the rFVIII B-domain to membrane attachment by transfecting several constructs containing increasing lengths of the B-domain into cells under serum free conditions. We found that 90% of rFVIII is attached to the cell membrane of the producing cell when the rFVIII variant contains a short B-domain (21 aa). By increasing the length of the B-domain the membrane attached fraction can be reduced to 50% of the total expressed rFVIII. Further, our studies show that the N-linked glycosylations within the B-domain have no influence on either total expression level or membrane attachment properties
U2 - 10.1016/j.jbiotec.2010.04.010
DO - 10.1016/j.jbiotec.2010.04.010
M3 - Journal article
C2 - 20438774
VL - 147
SP - 198
EP - 204
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 3-4
ER -
ID: 33343923