The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation

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Standard

The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation. / Clement, Christian A; Kristensen, Stine G; Møllgård, Kjeld; Pazour, Gregory J; Yoder, Bradley K; Larsen, Lars A; Christensen, Søren Tvorup.

I: Journal of Cell Science, Bind 122, Nr. Pt 17, 2009, s. 3070-82.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Clement, CA, Kristensen, SG, Møllgård, K, Pazour, GJ, Yoder, BK, Larsen, LA & Christensen, ST 2009, 'The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation', Journal of Cell Science, bind 122, nr. Pt 17, s. 3070-82. https://doi.org/10.1242/jcs.049676

APA

Clement, C. A., Kristensen, S. G., Møllgård, K., Pazour, G. J., Yoder, B. K., Larsen, L. A., & Christensen, S. T. (2009). The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation. Journal of Cell Science, 122(Pt 17), 3070-82. https://doi.org/10.1242/jcs.049676

Vancouver

Clement CA, Kristensen SG, Møllgård K, Pazour GJ, Yoder BK, Larsen LA o.a. The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation. Journal of Cell Science. 2009;122(Pt 17):3070-82. https://doi.org/10.1242/jcs.049676

Author

Clement, Christian A ; Kristensen, Stine G ; Møllgård, Kjeld ; Pazour, Gregory J ; Yoder, Bradley K ; Larsen, Lars A ; Christensen, Søren Tvorup. / The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation. I: Journal of Cell Science. 2009 ; Bind 122, Nr. Pt 17. s. 3070-82.

Bibtex

@article{5c6d3b30ad8d11debc73000ea68e967b,
title = "The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation",
abstract = "Defects in the assembly or function of primary cilia, which are sensory organelles, are tightly coupled to developmental defects and diseases in mammals. Here, we investigated the function of the primary cilium in regulating hedgehog signaling and early cardiogenesis. We report that the pluripotent P19.CL6 mouse stem cell line, which can differentiate into beating cardiomyocytes, forms primary cilia that contain essential components of the hedgehog pathway, including Smoothened, Patched-1 and Gli2. Knockdown of the primary cilium by Ift88 and Ift20 siRNA or treatment with cyclopamine, an inhibitor of Smoothened, blocks hedgehog signaling in P19.CL6 cells, as well as differentiation of the cells into beating cardiomyocytes. E11.5 embryos of the Ift88(tm1Rpw) (Ift88-null) mice, which form no cilia, have ventricular dilation, decreased myocardial trabeculation and abnormal outflow tract development. These data support the conclusion that cardiac primary cilia are crucial in early heart development, where they partly coordinate hedgehog signaling.",
author = "Clement, {Christian A} and Kristensen, {Stine G} and Kjeld M{\o}llg{\aa}rd and Pazour, {Gregory J} and Yoder, {Bradley K} and Larsen, {Lars A} and Christensen, {S{\o}ren Tvorup}",
year = "2009",
doi = "10.1242/jcs.049676",
language = "English",
volume = "122",
pages = "3070--82",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "The/Company of Biologists Ltd.",
number = "Pt 17",

}

RIS

TY - JOUR

T1 - The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation

AU - Clement, Christian A

AU - Kristensen, Stine G

AU - Møllgård, Kjeld

AU - Pazour, Gregory J

AU - Yoder, Bradley K

AU - Larsen, Lars A

AU - Christensen, Søren Tvorup

PY - 2009

Y1 - 2009

N2 - Defects in the assembly or function of primary cilia, which are sensory organelles, are tightly coupled to developmental defects and diseases in mammals. Here, we investigated the function of the primary cilium in regulating hedgehog signaling and early cardiogenesis. We report that the pluripotent P19.CL6 mouse stem cell line, which can differentiate into beating cardiomyocytes, forms primary cilia that contain essential components of the hedgehog pathway, including Smoothened, Patched-1 and Gli2. Knockdown of the primary cilium by Ift88 and Ift20 siRNA or treatment with cyclopamine, an inhibitor of Smoothened, blocks hedgehog signaling in P19.CL6 cells, as well as differentiation of the cells into beating cardiomyocytes. E11.5 embryos of the Ift88(tm1Rpw) (Ift88-null) mice, which form no cilia, have ventricular dilation, decreased myocardial trabeculation and abnormal outflow tract development. These data support the conclusion that cardiac primary cilia are crucial in early heart development, where they partly coordinate hedgehog signaling.

AB - Defects in the assembly or function of primary cilia, which are sensory organelles, are tightly coupled to developmental defects and diseases in mammals. Here, we investigated the function of the primary cilium in regulating hedgehog signaling and early cardiogenesis. We report that the pluripotent P19.CL6 mouse stem cell line, which can differentiate into beating cardiomyocytes, forms primary cilia that contain essential components of the hedgehog pathway, including Smoothened, Patched-1 and Gli2. Knockdown of the primary cilium by Ift88 and Ift20 siRNA or treatment with cyclopamine, an inhibitor of Smoothened, blocks hedgehog signaling in P19.CL6 cells, as well as differentiation of the cells into beating cardiomyocytes. E11.5 embryos of the Ift88(tm1Rpw) (Ift88-null) mice, which form no cilia, have ventricular dilation, decreased myocardial trabeculation and abnormal outflow tract development. These data support the conclusion that cardiac primary cilia are crucial in early heart development, where they partly coordinate hedgehog signaling.

U2 - 10.1242/jcs.049676

DO - 10.1242/jcs.049676

M3 - Journal article

C2 - 19654211

VL - 122

SP - 3070

EP - 3082

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - Pt 17

ER -

ID: 14828154