ThermoMouse: An In Vivo Model to Identify Modulators of UCP1 Expression in Brown Adipose Tissue
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Obesity develops when energy intake chronically exceeds energy expenditure. Because brown adiposetissue (BAT) dissipates energy in the form of heat, increasing energy expenditure by augmenting BAT-mediated thermogenesis may represent an approach to counter obesity and its complications. The ability of BAT to dissipate energy is dependent on expression of mitochondrial uncoupling protein 1 (UCP1). To facilitate the identification of pharmacological modulators of BAT UCP1 levels, which may have potential as antiobesity medications, we developed a transgenic model in which luciferase activity faithfully mimics endogenous UCP1 expression and its response to physiologic stimuli. Phenotypic screening of a library using cells derived from this model yielded a small molecule that increases UCP1 expression in brown fat cells and mice. Upon adrenergic stimulation, compound-treated mice showed increased energy expenditure. These tools offer an opportunity to identify pharmacologic modulators of UCP1 expression and uncover regulatory pathways that impact BAT-mediated thermogenesis. Pharmacological activation of brown adipose tissue (BAT) thermogenesis and energy dissipation, a process mediated by UCP1, may be useful to counter the energy imbalance that engenders obesity. Galmozzi etal. have developed an invivo model to monitor UCP1 expression in real time and identified a small molecule that increases UCP1 levels. Mice treated with this molecule show greater energy expenditure upon adrenergic stimulation. Discovery of compounds with this ability is an important stride toward enhancing BAT function in obese individuals.
Originalsprog | Engelsk |
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Tidsskrift | Cell Reports |
Vol/bind | 9 |
Udgave nummer | 5 |
Sider (fra-til) | 1584-1593 |
Antal sider | 10 |
ISSN | 2211-1247 |
DOI | |
Status | Udgivet - 2014 |
Eksternt udgivet | Ja |
Bibliografisk note
Funding Information:
We thank Haemin Hong, Kathleen Jay, Christophe Paillart, and Denis Glenn for assistance. Work was supported by NIH grants DK087853 and DK97441 to S.K. and DK099810 and CA179489 to E.S. S.K. acknowledges support from the DERC center grant ( DK63720 ), UCSF PBBR program , the Pew Charitable Trust , and PRESTO from Japan Science and Technology Agency . S.B.S. was supported by a fellowship from the Alfred Benzon Foundation and A.G. by fellowship 14POST18200019 from the American Heart Association .
Publisher Copyright:
© 2014 The Authors.
ID: 375199903