TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution

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Standard

TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution. / Samuelsen, Camilla O; Baraznenok, Vera; Khorosjutina, Olga; Spahr, Henrik; Kieselbach, Thomas; Holmberg, Steen; Gustafsson, Claes M.

I: Proceedings of the National Academy of Science of the United States of America, Bind 100, Nr. 11, 2003, s. 6422-6427.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Samuelsen, CO, Baraznenok, V, Khorosjutina, O, Spahr, H, Kieselbach, T, Holmberg, S & Gustafsson, CM 2003, 'TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution', Proceedings of the National Academy of Science of the United States of America, bind 100, nr. 11, s. 6422-6427. https://doi.org/10.1073/pnas.1030497100

APA

Samuelsen, C. O., Baraznenok, V., Khorosjutina, O., Spahr, H., Kieselbach, T., Holmberg, S., & Gustafsson, C. M. (2003). TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution. Proceedings of the National Academy of Science of the United States of America, 100(11), 6422-6427. https://doi.org/10.1073/pnas.1030497100

Vancouver

Samuelsen CO, Baraznenok V, Khorosjutina O, Spahr H, Kieselbach T, Holmberg S o.a. TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution. Proceedings of the National Academy of Science of the United States of America. 2003;100(11):6422-6427. https://doi.org/10.1073/pnas.1030497100

Author

Samuelsen, Camilla O ; Baraznenok, Vera ; Khorosjutina, Olga ; Spahr, Henrik ; Kieselbach, Thomas ; Holmberg, Steen ; Gustafsson, Claes M. / TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution. I: Proceedings of the National Academy of Science of the United States of America. 2003 ; Bind 100, Nr. 11. s. 6422-6427.

Bibtex

@article{7d840e2074c411dbbee902004c4f4f50,
title = "TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution",
abstract = "In Saccharomyces cerevisiae Mediator, a subgroup of proteins (Srb8, Srb9, Srb10, and Srb11) form a module, which is involved in negative regulation of transcription. Homologues of Srb10 and Srb11 are found in some mammalian Mediator preparations, whereas no clear homologues have been reported for Srb8 and Srb9. Here, we identify a TRAP240/ARC250 homologue in Schizosaccharomyces pombe and demonstrate that this protein, spTrap240, is stably associated with a larger form of Mediator, which also contains conserved homologues of Srb8, Srb10, and Srb11. We find that spTrap240 and Sch. pombe Srb8 (spSrb8) regulate the same distinct subset of genes and have indistinguishable phenotypic characteristics. Importantly, Mediator containing the spSrb8/spTrap240/spSrb10/spSrb11 subunits is isolated only in free form, devoid of RNA polymerase II. In contrast, Mediator lacking this module associates with the polymerase. Our findings provide experimental evidence for recent suggestions that TRAP230/ARC240 and TRAP240/ARC250 may indeed be the Srb8 and Srb9 homologues of mammalian Mediator. Apparently Srb8/TRAP230/ARC240, Srb9/TRAP240/ARC250, Srb10, and Srb11 constitute a conserved Mediator submodule, which is involved in negative regulation of transcription in all eukaryotes.",
author = "Samuelsen, {Camilla O} and Vera Baraznenok and Olga Khorosjutina and Henrik Spahr and Thomas Kieselbach and Steen Holmberg and Gustafsson, {Claes M}",
note = "Keywords: Amino Acid Sequence; Evolution, Molecular; Fungal Proteins; Molecular Sequence Data; Schizosaccharomyces; Sequence Homology, Amino Acid",
year = "2003",
doi = "10.1073/pnas.1030497100",
language = "English",
volume = "100",
pages = "6422--6427",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "11",

}

RIS

TY - JOUR

T1 - TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution

AU - Samuelsen, Camilla O

AU - Baraznenok, Vera

AU - Khorosjutina, Olga

AU - Spahr, Henrik

AU - Kieselbach, Thomas

AU - Holmberg, Steen

AU - Gustafsson, Claes M

N1 - Keywords: Amino Acid Sequence; Evolution, Molecular; Fungal Proteins; Molecular Sequence Data; Schizosaccharomyces; Sequence Homology, Amino Acid

PY - 2003

Y1 - 2003

N2 - In Saccharomyces cerevisiae Mediator, a subgroup of proteins (Srb8, Srb9, Srb10, and Srb11) form a module, which is involved in negative regulation of transcription. Homologues of Srb10 and Srb11 are found in some mammalian Mediator preparations, whereas no clear homologues have been reported for Srb8 and Srb9. Here, we identify a TRAP240/ARC250 homologue in Schizosaccharomyces pombe and demonstrate that this protein, spTrap240, is stably associated with a larger form of Mediator, which also contains conserved homologues of Srb8, Srb10, and Srb11. We find that spTrap240 and Sch. pombe Srb8 (spSrb8) regulate the same distinct subset of genes and have indistinguishable phenotypic characteristics. Importantly, Mediator containing the spSrb8/spTrap240/spSrb10/spSrb11 subunits is isolated only in free form, devoid of RNA polymerase II. In contrast, Mediator lacking this module associates with the polymerase. Our findings provide experimental evidence for recent suggestions that TRAP230/ARC240 and TRAP240/ARC250 may indeed be the Srb8 and Srb9 homologues of mammalian Mediator. Apparently Srb8/TRAP230/ARC240, Srb9/TRAP240/ARC250, Srb10, and Srb11 constitute a conserved Mediator submodule, which is involved in negative regulation of transcription in all eukaryotes.

AB - In Saccharomyces cerevisiae Mediator, a subgroup of proteins (Srb8, Srb9, Srb10, and Srb11) form a module, which is involved in negative regulation of transcription. Homologues of Srb10 and Srb11 are found in some mammalian Mediator preparations, whereas no clear homologues have been reported for Srb8 and Srb9. Here, we identify a TRAP240/ARC250 homologue in Schizosaccharomyces pombe and demonstrate that this protein, spTrap240, is stably associated with a larger form of Mediator, which also contains conserved homologues of Srb8, Srb10, and Srb11. We find that spTrap240 and Sch. pombe Srb8 (spSrb8) regulate the same distinct subset of genes and have indistinguishable phenotypic characteristics. Importantly, Mediator containing the spSrb8/spTrap240/spSrb10/spSrb11 subunits is isolated only in free form, devoid of RNA polymerase II. In contrast, Mediator lacking this module associates with the polymerase. Our findings provide experimental evidence for recent suggestions that TRAP230/ARC240 and TRAP240/ARC250 may indeed be the Srb8 and Srb9 homologues of mammalian Mediator. Apparently Srb8/TRAP230/ARC240, Srb9/TRAP240/ARC250, Srb10, and Srb11 constitute a conserved Mediator submodule, which is involved in negative regulation of transcription in all eukaryotes.

U2 - 10.1073/pnas.1030497100

DO - 10.1073/pnas.1030497100

M3 - Journal article

C2 - 12738880

VL - 100

SP - 6422

EP - 6427

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11

ER -

ID: 114327