Using quantitative PCR to Identify Kinesin-3 Genes that are Upregulated During Growth Arrest in Mouse NIH3T3 Cells
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Using quantitative PCR to Identify Kinesin-3 Genes that are Upregulated During Growth Arrest in Mouse NIH3T3 Cells. / Thorsteinsson, Rikke I.; Christensen, Søren T.; Pedersen, Lotte B.
Primary Cilia. red. / Roger D. Sloboda. Elsevier, 2009. s. 66-86 (Methods in Cell Biology, Bind 94).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Using quantitative PCR to Identify Kinesin-3 Genes that are Upregulated During Growth Arrest in Mouse NIH3T3 Cells
AU - Thorsteinsson, Rikke I.
AU - Christensen, Søren T.
AU - Pedersen, Lotte B.
PY - 2009
Y1 - 2009
N2 - Most cells in our body form a single primary cilium when entering growth arrest. During the past decade, a number of studies have revealed a key role for primary cilia in coordinating a variety of signaling pathways that control important cellular and developmental processes. Consequently, significant effort has been directed toward the identification of genes involved in ciliary assembly and function. Many candidate ciliary genes and proteins have been identified using large-scale "omics" approaches, including proteomics, transcriptomics, and comparative genomics. Although such large-scale approaches can be extremely informative, additional validation of candidate ciliary genes using alternative "small-scale" approaches is often necessary. Here we describe a quantitative PCR-based method that can be used to screen groups of genes for those that are upregulated during growth arrest in cultured mouse NIH3T3 cells and those that might have cilia-related functions. We employed this method to specifically search for mouse kinesin-3 genes that are upregulated during growth arrest and identified three such genes (Kif13A, Kif13B, and Kif16A). In principle, however, the method can be extended to identify other genes or gene families that are upregulated during growth arrest. 2009 Elsevier Inc. All rights reserved.
AB - Most cells in our body form a single primary cilium when entering growth arrest. During the past decade, a number of studies have revealed a key role for primary cilia in coordinating a variety of signaling pathways that control important cellular and developmental processes. Consequently, significant effort has been directed toward the identification of genes involved in ciliary assembly and function. Many candidate ciliary genes and proteins have been identified using large-scale "omics" approaches, including proteomics, transcriptomics, and comparative genomics. Although such large-scale approaches can be extremely informative, additional validation of candidate ciliary genes using alternative "small-scale" approaches is often necessary. Here we describe a quantitative PCR-based method that can be used to screen groups of genes for those that are upregulated during growth arrest in cultured mouse NIH3T3 cells and those that might have cilia-related functions. We employed this method to specifically search for mouse kinesin-3 genes that are upregulated during growth arrest and identified three such genes (Kif13A, Kif13B, and Kif16A). In principle, however, the method can be extended to identify other genes or gene families that are upregulated during growth arrest. 2009 Elsevier Inc. All rights reserved.
U2 - 10.1016/S0091-679X(08)94003-6
DO - 10.1016/S0091-679X(08)94003-6
M3 - Book chapter
C2 - 20362085
AN - SCOPUS:77953680617
SN - 978-0-12-375024-2
T3 - Methods in Cell Biology
SP - 66
EP - 86
BT - Primary Cilia
A2 - Sloboda, Roger D.
PB - Elsevier
ER -
ID: 250604653